Retinoic-acid-induced limb-reduction defects: perturbation of zones of programmed cell death as a pathogenetic mechanism.
Retinoic-acid-induced limb-reduction defects: perturbation of zones of programmed cell death as a pathogenetic mechanism.
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视黄酸诱导的肢体减少缺陷：程序性细胞死亡区域的扰动作为发病机制。
DOI：
10.1002/tera.1420400210
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Sulik,KK
中科院分区：
文献类型：
--
作者：
Alles,AJ;Sulik,KK
妊娠C57 B1/6 J小鼠在妊娠第11.0天接受100 mg/kg体重的全反式维甲酸芝麻油溶液给药。在妊娠第18天收获的活胎中，通过大体检查和骨骼染色确定100%的胎仔存在四肢中肢缺陷。接受芝麻油治疗的对照胎仔没有明显的肢体畸形。在处理后12小时收获一些处理和对照胚胎，并使用超活体染色硫酸尼罗蓝和亚甲蓝和酸性品红染色的组织切片检查细胞死亡模式。视黄酸诱导的肢体核心细胞死亡总是与程序性细胞死亡区相关，如在可比较阶段的对照胚胎中所见。这一点，在concet与以前的研究表明，过度的细胞死亡的程序性细胞死亡，随后的畸形相关的区域，使我们得出结论，mesomelic畸形的发病机制与程序性细胞死亡的现象有一个主要的关联。
Pregnant C57B1/6J mice were treated with 100 mg/kg body weight of all‐trans retinoic acid in sesame oil on day 11.0 of gestation. Among the live fetuses harvested on day 18 of gestation, 100% had mesomelic defects of the limbs as determined by gross examination and skeletal staining. Control fetuses treated with sesame oil had no observable limb malformations. Some treated and control embryos were harvested 12 hr after treatment and examined for patterns of cell death by using the supravital stain Nile blue sulphate and methylene‐blue‐ and acid‐fuchsin‐stained histological sections. Retinoic‐acid‐induced cell death in the core of the limb was always associated with the zones of programmed cell death as seen in control embryos of comparable stages. This, in concet with previous studies demonstrating excessive cell death in regions of programmed cell death that correlated with subsequent malformations, leads us to conclude that the pathogenesis of mesomelic malformations has a primary association with the phenomenon of programmed cell death.