Unique microRNA molecular profiles in lung cancer diagnosis and prognosis
Unique microRNA molecular profiles in lung cancer diagnosis and prognosis
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DOI：
10.1016/j.ccr.2006.01.025
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发表时间：
2006-03-01
期刊：
影响因子：
50.3
通讯作者：
Harris, CC
中科院分区：
文献类型：
--
作者：
Yanaihara, N;Caplen, N;Harris, CC
检测肺癌组织中microRNA（miRNA）的表达谱，探讨miRNA在肺癌发生中的作用。miRNA微阵列分析确定了统计学上独特的特征，可以区分肺癌和非癌肺组织以及肿瘤组织学上不同的分子特征。miRNA表达谱与肺腺癌的生存相关，包括那些分类为疾病1期的腺癌。hsa-mir-155的单变量分析和多变量分析表明，hsa-mir-155高表达和hsa-let-7a-2低表达与生存率低相关。通过前体miRNA的实时RT-PCR分析证实了miRNA表达对结果的影响，并与一组独立的腺癌进行了交叉验证。这些结果表明，miRNA表达谱是肺癌的诊断和预后标志物。
MicroRNA (miRNA) expression profiles for lung cancers were examined to investigate miRNA's involvement in lung carcinogenesis. miRNA microarray analysis identified statistical unique profiles, which could discriminate lung cancers from noncancerous lung tissues as well as molecular signatures that differ in tumor histology. miRNA expression profiles correlated with survival of lung adenocarcinomas, including those classified as disease stage 1. High hsa-mir-155 and low hsa-let-7a-2 expression correlated with poor survival by univariate analysis as well as multivariate analysis for hsa-mir-155. The miRNA expression signature on outcome was confirmed by real-time RT-PCR analysis of precursor miRNAs and crossvalidated with an independent set of adenocarcinomas. These results indicate that miRNA expression profiles are diagnostic and prognostic markers of lung cancer.