Cellular behavior in embryogenic and non-embryogenic sugar beet calluses
Cellular behavior in embryogenic and non-embryogenic sugar beet calluses
复制标题
DOI：
10.1079/ivp2005641
复制
发表时间：
2005-07-01
影响因子：
2.6
通讯作者：
Taha, RM
中科院分区：
文献类型：
--
作者：
Moghaddam, BE;Taha, RM
本研究讨论了两种甜菜愈伤组织（即胚胎发生组织和非胚胎发生组织）的细胞学研究结果。通过在含有两种激素组合的 Murashige 和 Skoog 培养基上培养次生叶来产生愈伤组织。将愈伤组织转移到新鲜培养基上一周后，使用电子显微镜和图像分析仪观察它们的细胞。观察结果表明，两种愈伤组织类型的细胞在细胞结构和各种细胞器方面存在较大差异。值得注意的是，与胚胎发生细胞相比，非胚胎发生细胞中存在大量多倍化、粗面内质网、多核糖体、多核仁和不完整的细胞壁以及异常分配。相反，在胚胎发生细胞中发现了细胞质的空泡、完美的细胞壁和分隔结构以及高比例的细胞核/细胞质面积。
The present study discusses the results of cytological studies of two kinds of sugar beet callus, i.e., embryogenic and non-embryogenic tissues. The calluses were produced through culture of secondary leaves on Murashige and Skoog medium containing two hormone combinations. One week after transfer of calluses onto fresh medium, their cells were viewed using electron microscopy and an image analyzer. Observations showed that cells of the two callus types had considerable differences in cell structure and various organelles. Of note were the high amount of polyploidization, rough endoplasmic reticulum, polysome, poly-nucleolus, and incomplete cell wall together with abnormal partitioning in non-embryogenic cells, as compared to embryogenic cells. In contrast, vacuolation of cytoplasm, perfect cell wall and partitioning structure, and the high proportion of nucleus/cytoplasm area were recognized in embryogenic cells.