Effects of Fiber Diameter and Spacing Size of an Artificial Scaffold on the In Vivo Cellular Response and Tissue Remodeling
Effects of Fiber Diameter and Spacing Size of an Artificial Scaffold on the In Vivo Cellular Response and Tissue Remodeling
复制标题
人工支架纤维直径和间距尺寸对体内细胞反应和组织重塑的影响
DOI：
10.1021/acsabm.1c00572
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发表时间：
2021
影响因子：
4.7
通讯作者：
A Kawauchi
中科院分区：
文献类型：
--
作者：
T Horii;H Tsujimoto;A Hagiwara;N Isogai;Y Sueyoshi;Y Oe;S Kageyama;T Yoshida;K Kobayashi;H Minato;J Ueda;H Ichikawa;A Kawauchi
通过模拟细胞外基质，非织造织物可以理想地用作组织工程应用的支架，并且已经在体外对其纤维直径和纤维间距（间距尺寸）进行了表征。我们按时间顺序研究了这些织物对细胞反应和组织重塑的体内影响。四种聚乙醇酸非织造布将纤维（纤维直径分别为0.7、0.9、3.0和16.2 μm，间距分别为2.0、19.3、19.0和825.4 μm的织物-0.7、织物-0.9、织物-3和织物-16）植入大鼠背部，并从第3天至第70天进行组织学和免疫组织化学分析。对于Fabric-0.7，炎性细胞（主要是M1巨噬细胞）和具有III型胶原的肌成纤维细胞主要积聚在织物的表面上，并且最初没有渗透到织物内部，这可能是由于狭窄的纤维空间。然后随着织物的降解出现大量I型胶原蛋白的形成，最后，重塑的组织变成致密的瘢痕。对于Fabric-0.9和Fabric-3，最初在织物的所有层中观察到炎性细胞（主要是M2巨噬细胞）。然后观察到I型胶原蛋白的轻度增加，肌成纤维细胞很少，并且重塑的组织最终显示出相对较少的瘢痕，具有由织物诱导的足够厚度的组织。对于Fabric-16，炎性细胞（主要是M1巨噬细胞）最初沿着许多肌成纤维细胞浸润到织物的所有层中，特别是在孔中。最近，由于织物的缓慢降解，随着织物的大幅收缩，注意到大量I型胶原蛋白的形成，并且重塑的组织最终变成致密的疤痕。这些发现表明，优化人工支架的间距大小以及纤维直径可以控制细胞反应和组织重塑，并促进有利的组织再生而不形成瘢痕。
By mimicking the extracellular matrix, nonwoven fabrics can function as scaffolds for tissue engineering application ideally, and they have been characterized regarding their fiber diameter and fiber spacing (spacing size) in vitro. We chronologically examined the in vivo effects of these fabrics on the cellular response and tissue remodeling. Four types of nonwoven polyglycolic acid fabrics (Fabric-0.7, Fabric-0.9, Fabric-3, and Fabric-16 with fiber diameters of 0.7, 0.9, 3.0, and 16.2 μm and spacing sizes of 2.0, 19.3, 19.0, and 825.4 μm, respectively) were implanted into the rat dorsum and subjected to histologic and immunohistochemical analyses from day 3 to 70. With Fabric-0.7, inflammatory cells (mainly M1 macrophages) and myofibroblasts with collagen type III accumulated mainly on the surface of the fabric and did not infiltrate inside the fabric initially, likely due to the narrow fiber space. Massive formation of collagen type I then appeared with the degradation of the fabrics, and finally, the remodeled tissue turned into a dense scar. With Fabric-0.9 and Fabric-3, inflammatory cells (predominantly M2 macrophages) were seen in all layers of the fabric initially. A mild increase in collagen type I was then seen, with few myofibroblasts, and the remodeled tissue ultimately showed a relatively little scar with an adequate thickness of the tissue induced by the fabrics. With Fabric-16, inflammatory cells (predominantly M1 macrophages) infiltrated into all layers of the fabric initially along with many myofibroblasts, especially in the hole. Lately, massive formation of collagen type I was noted due to the slow degradation of the fabric, with the shrinking of the fabric substantially, and the remodeled tissue finally turned to a dense scar. These findings suggest that optimizing the spacing size as well as the fiber diameter of artificial scaffolds may control the cellular response and tissue remodeling and facilitate favorable tissue regeneration without scar formation.