Transcranial pulse current stimulation improves the locomotor function in a rat model of stroke.
Transcranial pulse current stimulation improves the locomotor function in a rat model of stroke.
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DOI：
10.4103/1673-5374.301018
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发表时间：
2021-07
影响因子：
6.1
通讯作者：
Shan CL
中科院分区：
文献类型：
--
作者：
Wang WJ;Zhong YB;Zhao JJ;Ren M;Zhang SC;Xu MS;Xu ST;Zhang YJ;Shan CL
已有研究表明，经颅脉冲电流刺激（tPCS）可以增加脑神经可塑性，改善患者的运动功能。然而，这种效应背后的确切机制仍不清楚。在本研究中，通过阻塞右侧大脑中动脉建立的中风大鼠模型经受tPCS，每天20分钟，连续7天。与干预前相比，tPCS可显著降低脑卒中大鼠Bederson评分，增加患肢足印面积，减少患肢站立时间。免疫荧光染色和western blot检测显示，tPCS显著增加缺血半暗带周围微管相关蛋白2和生长相关蛋白43的表达。这一发现表明，tPCS可以通过调节缺血半暗带周围微管相关蛋白-2和生长相关蛋白-43的表达来改善中风大鼠的运动功能。本研究为脑卒中后运动功能障碍的临床治疗提供了新的方法，为tPCS的临床应用提供了理论依据。本研究于2019年2月22日获得中国上海中医药大学动物使用与管理委员会批准（批准号：PZSHUTCM 190315003）。
Previous studies have shown that transcranial pulse current stimulation (tPCS) can increase cerebral neural plasticity and improve patients’ locomotor function. However, the precise mechanisms underlying this effect remain unclear. In the present study, rat models of stroke established by occlusion of the right cerebral middle artery were subjected to tPCS, 20 minutes per day for 7 successive days. tPCS significantly reduced the Bederson score, increased the foot print area of the affected limbs, and reduced the standing time of affected limbs of rats with stroke compared with that before intervention. Immunofluorescence staining and western blot assay revealed that tPCS significantly increased the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra. This finding suggests that tPCS can improve the locomotor function of rats with stroke by regulating the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra. These findings may provide a new method for the clinical treatment of poststroke motor dysfunction and a theoretical basis for clinical application of tPCS. The study was approved by the Animal Use and Management Committee of Shanghai University of Traditional Chinese Medicine of China (approval No. PZSHUTCM190315003) on February 22, 2019.