VDAC is a conserved element of death pathways in plant and animal systems
VDAC is a conserved element of death pathways in plant and animal systems
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DOI：
10.1016/s0167-4889(03)00102-2
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发表时间：
2003-09-23
影响因子：
5.1
通讯作者：
Mathew, MK
中科院分区：
文献类型：
--
作者：
Godbole, A;Varghese, J;Mathew, MK
程序性细胞死亡（PCD）是植物生命的重要组成部分，尽管其潜在机制不像动物那样被很好地理解。在动物细胞中，电压依赖性阴离子通道（voltage-dependent阴离子channel， VDAC）是一种主要的线粒体外膜转运蛋白，通过参与膜间空间蛋白的释放，在细胞凋亡过程中发挥重要作用。为了通过研究哺乳动物细胞凋亡中可能成分的功能来表征植物PCD通路，我们在Jurkat t细胞系中过表达水稻VDAC （osVDAC4）。osVDAC4过表达诱导细胞凋亡，可被Bcl-2和VDAC抑制剂DIDS阻断。通过DIDS和己糖激酶II （HxKII）修饰Jurkat细胞内源性VDAC功能抑制线粒体介导的凋亡途径。最后，我们发现DIDS还能消除黄瓜子叶热诱导的PCD。我们的数据表明，VDAC是植物和动物细胞死亡机制的保守线粒体元件。(C) 2003 Elsevier B.V.版权所有
Programmed cell death (PCD) is very much a part of plant life, although the underlying mechanisms are not so well understood as in animals. In animal cells, the voltage-dependent anion channel (VDAC), a major mitochondrial outer membrane transporter, plays an important role in apoptosis by participating in the release of intermembrane space proteins. To characterize plant PCD pathways by investigating the function of putative components in a mammalian apoptotic context, we have overexpressed a rice VDAC (osVDAC4) in the Jurkat T-cell line. Overexpression of osVDAC4 induces apoptosis, which can be blocked by Bcl-2 and the VDAC inhibitor DIDS. Modifying endogenous VDAC function by DIDS and hexokinase II (HxKII) in Jurkat cells inhibits mitochondria-mediated apoptotic pathways. Finally, we show that DIDS also abrogates heat-induced PCD in cucumber cotyledons. Our data suggest that VDAC is a conserved mitochondrial element of the death machinery in both plant and animal cells. (C) 2003 Elsevier B.V. All rights reserved.