Evaluation of Placental VEGFA mRNA Expression in Preeclampsia: A Case Control Study
Evaluation of Placental VEGFA mRNA Expression in Preeclampsia: A Case Control Study
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DOI：
10.1007/s13224-018-1128-2
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发表时间：
2019-04-01
影响因子：
0.7
通讯作者：
Radhakrishnan, Gita
中科院分区：
文献类型：
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作者：
Agarwal, Rachna;Kumari, Neelam;Radhakrishnan, Gita
我们的病例对照研究的目的是确定VEGFA mRNA在先兆子痫（PE）与单纯妊娠胎盘中的表达，以进一步阐明其在妊娠高血压疾病中的差异表达。研究设计PE组被细分为严重和非严重;那些有或没有HELLP综合征和胎盘VEGFA特征进行了比较，这些队列。此外，还记录了新生儿和母亲的结局。胎盘VEGFA的定量使用定量实时PCR和结果表示为倍数changed.ResultsOut的42例PE的情况下，23例（55%）是非严重和19例（45%）是严重的PE。在19例重度PE患者中，8例（42%）为HELLP综合征（完全HELLP），其余11例（58%）为非HELLP重度PE。与对照组相比，PE、HELLP、非HELLP、重度PE、非重度PE的真实倍数变化分别为-2.186、-13.333、-6.698、-8.950和1.466。结论我们的结果显示，与简单的对照组相比，PE胎盘中VEGFA表达降低。在非重度PE中VEGFA的初始增加和随后HELLP的显著降低的发现加强了失代偿VEGF在PE中起主要作用的现有假设。
ObjectiveThe aim of our case-control study was to determine expression of VEGFA mRNA in placentae of preeclamsia (PE) versus uncomplicated pregnancy to further clarify its differential expression in pregnancy hypertensive disorders.Study DesignThe PE group was subdivided into severe and non-severe; those with or without HELLP syndrome and placental VEGFA characteristics were compared for these cohorts. Additionally, the neonatal and maternal outcomes were recorded. The quantification of placental VEGFA was done using quantitative real-time PCR and results were expressed as fold change.ResultsOut of 42 PE cases, 23 (55%) were non-severe and 19 cases (45%) were severe PE. Out of 19 severe PE patients, 8 (42%) were HELLP syndrome (complete HELLP) and remaining 11 (58%) were non-HELLP severe PE. Compared to controls, the true fold change in PE, HELLP, non-HELLP, severe PE, non-severe PE was -2.186, -13.333, -6.698, -8.950 and 1.466, respectively.ConclusionsOur results showed a lowered VEGFA expression in PE placentae compared to uncomplicated controls. The finding of initial increase of VEGFA in non-severe PE and subsequent marked lowering in HELLP strengthens the existing hypothesis of decompensated VEGF being a major role player in PE.