Isolation and characterization of an autonomously replicating sequence from Ustilago maydis.
Isolation and characterization of an autonomously replicating sequence from Ustilago maydis.
复制标题
玉米黑粉菌自主复制序列的分离和表征。
DOI：
10.1128/mcb.8.9.3703-3709.1988
复制
发表时间：
1988
影响因子：
5.3
通讯作者：
Holloman,WK
中科院分区：
文献类型：
--
作者：
Tsukuda,T;Carleton,S;Fotheringham,S;Holloman,WK
DNA fragments that function as autonomously replicating sequences (ARSs) have been isolated fromUstilago maydis. When inserted into an integrative transforming vector, the fragments increased the frequency ofU. maydistransformation several-thousandfold. ARS-containing plasmids were transmitted inU. maydisas extrachromosomal elements through replication. They were maintained at a level of about 25 copies per cell but were mitotically unstable. One ARS characterized in detail, which we called UARS1, was localized to a 1.7-kilobase fragment. UARS1 contained a cluster of active sequences. This element could be reduced further into three separate subfragments, each of which retained ARS activity. The smallest one was 383 base pairs (bp) long. Although not active itself in yeast, this small fragment contained seven 8-bp direct repeats, two contiguous 30-bp direct repeats, and five 11-bp units in both orientations with sequences similar but not identical to the consensus sequence found to be crucial for ARS activity inSaccharomyces cerevisiae.
