Differential antigen expression during metamorphosis in the tripartite olfactory system of the African clawed frog, Xenopus laevis.
Differential antigen expression during metamorphosis in the tripartite olfactory system of the African clawed frog, Xenopus laevis.
复制标题
非洲爪蛙（Xenopus laevis）变态过程中三方嗅觉系统的差异抗原表达。
DOI：
10.1007/s004410051366
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发表时间：
1999
影响因子：
3.6
通讯作者：
Burd,GD
中科院分区：
文献类型：
--
作者：
Petti,MA;Matheson,SF;Burd,GD
成年非洲爪蟾（Xenopus laevis）的嗅觉上皮位于三个独立的鼻腔中：主腔、中腔和鼻峰鼻器官。这些空腔中的感觉上皮具有不同的细胞特征，并在变态过程中产生生理和行为功能。最值得注意的是，中腔是从头形成的，主腔由暴露于水的幼虫感觉上皮转变为暴露于空气的成体嗅觉上皮。为了更清楚地了解这种可塑性的细胞性质，我们对x嗅觉系统中产生的染色模式进行了表征。一种新的单克隆抗体，抗e7。在胚胎发育后期，嗅上皮首次被抗e7染色。在变态过程中，嗅觉神经横断消除了所有染色，表明染色与成熟或接近成熟的嗅觉受体神经元有关。抗体在整个发育过程和成人中弥漫性地染色犁鼻器。在幼虫的主腔中，嗅觉受体神经元染色明亮，但这种细胞染色在变态后消失。然而，在成人中，来自主腔的鲍曼氏腺的粘液被强烈染色。在整个发育过程和成年期，中腔与幼虫主腔具有相同的染色特征。因此，E7抗体可以区分嗅上皮的三个区域，可以测量感觉上皮的体积，并且可以作为变态过程中感觉上皮变化的良好标记。
In the adult African clawed frog,Xenopus laevis, olfactory epithelium is housed in three separate nasal cavities: the principal cavity, the middle cavity, and the vomeronasal organ. The sensory epithelium in each of these cavities has distinct cellular features, and presumed physiological and behavioral functions, which arise during metamorphosis. Most notably, the middle cavity is formed de novo, and the principal cavity is transformed from a larval sensory epithelium with water exposure to an adult olfactory epithelium with air exposure. To understand the cellular nature of this plasticity more clearly, we characterized the staining patterns generated in the olfactory system ofX. laeviswith a new monoclonal antibody, anti-E7. The olfactory epithelium is first stained with anti-E7 during late embryonic development. Transection of the olfactory nerves during metamorphosis eliminates all staining and indicates that the staining is associated with mature or nearly mature olfactory receptor neurons. The antibody diffusely stains the vomeronasal organ throughout development and in adults. In the larval principal cavity, the olfactory receptor neurons are brightly stained, but this cellular staining is lost after metamorphosis. The mucus from Bowman’s glands in the principal cavity, however, is intensely stained in adults. The middle cavity, throughout development and in adulthood, has the same staining characteristics as the larval principal cavity. Thus, the E7 antibody can distinguish the three areas of the olfactory epithelium, allowing measurement of sensory epithelium volume, and serves as an excellent marker for the changes in the sensory epithelium that occur during metamorphosis.