Biochar and metal-immobilizing Serratia liquefaciens CL-1 synergistically reduced metal accumulation in wheat grains in a metal-contaminated soil.
Biochar and metal-immobilizing Serratia liquefaciens CL-1 synergistically reduced metal accumulation in wheat grains in a metal-contaminated soil.
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DOI：
10.1016/j.scitotenv.2020.139972
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发表时间：
2020-06
期刊：
影响因子：
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通讯作者：
Cheng Cheng-Cheng;Hui Han;Yaping Wang;Ru Wang;Linyan He;X. Sheng
中科院分区：
文献类型：
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作者：
Cheng Cheng-Cheng;Hui Han;Yaping Wang;Ru Wang;Linyan He;X. Sheng
生物炭和金属固定细菌在降低植物对金属的吸收方面起着重要作用。然而，很少有研究表明，生物炭和金属固定细菌的协同效应，减少金属在小麦籽粒中的积累和潜在的机制。研究了生物炭、金属固定化沙雷氏菌CL-1和生物炭+ CL-1对小麦（Triticum aestivumL.）苏麦-188）和田间条件下参与的机制进行了表征。与对照相比，生物炭、CL-1和生物炭+ CL-1分别降低小麦籽粒Cd和Pb含量17- 25%、24- 27%和45-55%，降低根际土壤有效态Cd和Pb含量14- 33%、13- 38%和27- 57%。生物炭、CL-1和生物炭+ CL-1增加了土壤pH值。基于qPCR分析，与对照相比，CL-1和生物炭+ CL-1使根际土壤中腐胺含量分别增加了93%和150%，bacterialaguA基因拷贝数分别增加了30%和44%。此外，生物炭+ CL-1降低了Cd和Pb的生物富集和转运因子的23-33%相比，控制。与对照相比，CL-1显著增加了金属污染土壤溶液的pH值，降低了水溶性Cd和Pb浓度（18-44%）。结果表明，生物炭和CL-1在降低小麦籽粒中Cd、Pb积累方面具有协同效应。这些研究结果表明，生物炭加CL-1降低小麦籽粒金属吸收，通过减少金属的可用性和易位从根到籽粒，增加pH值，腐胺生产，andaguAgene丰度，他们强调了开发一种有效的技术，减少小麦籽粒金属吸收的可能性，使用生物炭加金属固定化细菌在金属污染的土壤中。
Biochar and metal-immobilizing bacteria play an important role in reducing the metal uptake of plants. However, little research has characterized the synergistic effects of biochar and metal-immobilizing bacteria on reducing metal accumulation in wheat grains and the underlying mechanisms. In this study, the effects of biochar, metal-immobilizingSerratia liquefaciensCL-1, and biochar + CL-1 on grain Cd and Pb uptake in wheat (Triticum aestivumL. Sumai-188) and the mechanisms involved under field conditions were characterized. Biochar, CL-1, and biochar + CL-1 reduced wheat grain Cd and Pb contents by 17–25%, 24–27%, and 45–55% and reduced the available Cd and Pb contents in the rhizosphere soils by 14–33%, 13–38%, and 27–57%, respectively, compared with the controls. Biochar, CL-1, and biochar + CL-1 increased soil pH values. CL-1 and biochar + CL-1 increased putrescine contents by 93% and 150% and bacterialaguAgene copy numbers by 30% and 44%, respectively, in the rhizosphere soils compared to the controls based on qPCR analysis. Furthermore, biochar + CL-1 reduced the Cd and Pb bioconcentration and translocation factors by 23–33% compared to the controls. CL-1 significantly increased the pH and reduced water-soluble Cd and Pb concentrations (18–44%) in the metal-contaminated soil solution compared to the controls. The results showed a synergistic effect of biochar and CL-1 on the reduction of Cd and Pb accumulation in wheat grains. These findings suggested that biochar plus CL-1 reduced wheat grain metal uptake by reducing metal availability and translocation from the roots to grains and increasing pH levels, putrescine production, andaguAgene abundance, and they highlight the possibility of developing an effective technique for reducing the metal uptake of wheat grains using biochar plus metal-immobilizing bacteria in metal-contaminated soils.