Choline acetyltransferase and acetylcholinesterase in the normal, developing and regenerating newt retinas
Choline acetyltransferase and acetylcholinesterase in the normal, developing and regenerating newt retinas
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DOI：
10.1016/s0165-3806(99)00080-2
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发表时间：
1999-08-05
期刊：
影响因子：
--
通讯作者：
Saito, T
中科院分区：
文献类型：
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作者：
Cheon, EW;Saito, T
胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）的存在下，证明在成年蝾螈视网膜使用免疫细胞化学和组织化学技术。在内丛状层（IPL）内，在LPL总厚度（100%）的0-15%和45-60%的相对深度处检测到两条ChAT阳性条带。AChE阳性条带约占强脉冲光宽度的0-60%，在强脉冲光内20-40%深度处有一条较强的AChE阳性条带。本地化的最大ChAT和AChE活性并不完全相同，在IPL的成熟视网膜。为了阐明视网膜再生是否遵循视网膜发育中发生的细胞分化步骤的相同序列，我们研究了发育和再生视网膜中胆碱能神经元和AChE活性的出现的时间过程。ChAT阳性细胞首先在视网膜中检测到，就在发育和再生视网膜中IPL的形态发育之前或开始时。乙酰胆碱酯酶活性首先变得可检测到的位于最近的层的视网膜的胞体之前，可以检测到的ChAT阳性细胞和前IPL开发的发展和再生的视网膜。在随后的发展和再生，外丛状层，IPL，和靠近IPL两侧的胞体成为乙酰胆碱酯酶阳性。事实上，在再生过程中的ChAT和乙酰胆碱酯酶分子的外观的时间过程中观察到的发展过程中表明，共同的机制可能控制蝾螈视网膜的发展和再生。(C)1999 Elsevier Science B. V.保留所有权利。
The presence of the choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) was demonstrated in the adult newt retina using immunocytochemical and histochemical techniques. Within the inner plexiform layer (IPL), two ChAT-positive bands were detected at relative depths of 0-15% and 45-60% of the total thickness (100%) of the LPL. AChE-positive band occupied approximately 0-60% of the IPL width with an intensive AChE-positive band at a depth of 20-40% within the IPL. Localizations of maximum ChAT and AChE activity were not exactly the same in the IPL of the mature retina. To elucidate whether retinal regeneration follows the same sequence of cellular differentiation steps that occur in retinal development, we examined the time course of appearance of the cholinergic neurons and AChE activity in both developing and regenerating retinas. The ChAT-positive cells were first detected in the retina just before or at the beginning of the morphological development of the IPL in both developing and regenerating retinas. AChE activity first became detectable in somata located at the most proximal layer of the retina before the ChAT-positive cells could be detected and well before the IPL developed in both developing and regenerating retinas. During subsequent development and regeneration, the outer plexiform layer, the IPL, and somata close to either side of the IPL became AChE-positive. The fact that the time course of the appearance of ChAT and AChE molecules during regeneration was similar to that observed during development suggests that common mechanisms may control both the development and the regeneration of the newt retina. (C) 1999 Elsevier Science B.V. All rights reserved.