Three-Dimensional Analysis of Chloroplast Structures Associated with Virus Infection
Three-Dimensional Analysis of Chloroplast Structures Associated with Virus Infection
复制标题
与病毒感染相关的叶绿体结构的三维分析
DOI：
10.1104/pp.17.00871
复制
发表时间：
2018-01-01
期刊：
影响因子：
7.4
通讯作者：
Zhang, Yongliang
中科院分区：
文献类型：
--
作者：
Jin, Xuejiao;Jiang, Zhihao;Zhang, Yongliang
叶绿体是一种多功能的细胞器，其形态受环境胁迫的影响。虽然类囊体膜的三维（3D）结构已被报道，在压力下的叶绿体的三维可视化还没有探索。本研究以大麦条纹花叶病毒（BSMV）为材料，利用电子断层扫描技术，观察了病毒感染过程中叶绿体结构的变化。分析表明，重塑的叶绿体膜，其特征在于外膜内陷小球在内膜衍生包的集群。细胞质内陷（CI）的不同形态是明显的，在周边和不同大小的开口连接CI细胞质的小球。这些病毒成分的免疫电镜证实，异常的膜结构的网站BSMV复制。BSMV α a复制蛋白定位于叶绿体表面，并在引发叶绿体膜重排中起重要作用。总之，我们的结果揭示了BSMV感染诱导的叶绿体的三维结构。这些发现有助于我们理解叶绿体形态变化的压力条件下，在装配过程中的植物（+）RNA病毒复制复合物。
Chloroplasts are multifunctional organelles whose morphology is affected by environmental stresses. Although the three-dimensional (3D) architecture of thylakoid membranes has been reported previously, a 3D visualization of chloroplast under stress has not been explored. In this work, we used a positive-strand RNA ((+) RNA) virus, barley stripe mosaic virus (BSMV) to observe chloroplast structural changes during infection by electron tomography. The analyses revealed remodeling of the chloroplast membranes, characterized by the clustering of outer membrane-invaginated spherules in inner membrane-derived packets. Diverse morphologies of cytoplasmic invaginations (CIs) were evident with spherules at the periphery and different sized openings connecting the CIs to the cytoplasm. Immunoelectron microscopy of these viral components verified that the aberrant membrane structures were sites for BSMV replication. The BSMV alpha a replication protein localized at the surface of the chloroplasts and played a prominent role in eliciting chloroplast membrane rearrangements. In sum, our results have revealed the 3D structure of the chloroplasts induced by BSMV infection. These findings contribute to our understanding of chloroplast morphological changes under stress conditions and during assembly of plant (+) RNA virus replication complexes.