Coronary flow by left atrial and left ventricular microsphere injection in the rat.
Coronary flow by left atrial and left ventricular microsphere injection in the rat.
复制标题
大鼠左心房和左心室微球注射的冠状动脉血流。
DOI：
10.1152/ajpheart.1990.259.2.h635
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Ferguson,JL
中科院分区：
文献类型：
--
作者：
Schrock,GD;Krahmer,RL;Ferguson,JL
先前的研究已经报道了与微球注射到大鼠的左心房（LA）中相比，通过微球注射到左心室（LV）中获得的冠状动脉血流量（CBF）测量值的显著更高的变异性。出现了一些问题，是否有一些变异性可能是由于连续的，而不是同时注射在两个网站，从而在不同的心血管状态的测量。为了解决这个问题，我们测量心输出量（CO）和CBF以及其他全身器官血流量采用同时注射两个不同的放射性标记的微球到左心房和左心室的清醒大鼠。使用该技术，测得LA和LV注射部位的CBF值（ml.min-1. g-1）分别为5.45 +/- 0.43和5.24 +/- 0.46（所有值均报告为平均值+/- SE）。LA的CO值（ml/min）为67.5 +/- 3.5，LV为67.6 +/- 3.4。使用示踪微球方法对左心房和左心室的CBF、CO和其他全身器官血流量进行配对测量没有显著差异。LA和LV注射的这些测量值的所有变异性均无显著差异。我们的手术没有显著改变生理参数，如心率、平均动脉压、红细胞压积或血气。这些发现表明，在我们的大鼠模型中，通过LV微球注射进行的测量不仅足以确定远端床的全身血流量，而且提供的冠状动脉血流量数据的变异性与左心房注射没有显著差异。
Previous studies have reported significantly higher variability in coronary blood flow (CBF) measurements obtained by microsphere injection into the left ventricle (LV) as compared with microsphere injection into the left atrium (LA) of the rat. Questions have arisen concerning whether or not some of the variability may have been due to successive rather than simultaneous injections at the two sites, thereby giving measurements at different cardiovascular states. To address this question, we measured cardiac output (CO) and CBF as well as other systemic organ blood flows by employing simultaneous injection of two different sets of radiolabeled microspheres into the left atrium and left ventricle of semiconscious rats. Using this technique, CBF values (ml.min-1.g-1) of 5.45 +/- 0.43 and 5.24 +/- 0.46 for LA and LV injection sites, respectively, were measured (all values reported as means +/- SE). CO values (ml/min) of 67.5 +/- 3.5 for LA and 67.6 +/- 3.4 for LV were obtained. Paired left atrial and left ventricular measurements of CBF, CO, and other systemic organ blood flows using tracer microsphere methodology were not significantly different. All variabilities in these measurements by LA and LV injection were not significantly different. Our procedure did not significantly alter physiological parameters such as heart rate, mean arterial pressure, hematocrit, or blood gases. These findings indicate that, in our rat model, measurements made by LV microsphere injection are not only adequate for determining systemic blood flow at distal beds but provide coronary blood flow data with variability not significantly different from that of left atrial injection.