Bioadsorption of Rare Earth Elements through Cell Surface Display of Lanthanide Binding Tags.
Bioadsorption of Rare Earth Elements through Cell Surface Display of Lanthanide Binding Tags.
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通过细胞表面显示镧系元素结合标签来生物吸附稀土元素。
DOI：
10.1021/acs.est.5b06129
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发表时间：
2016-03-01
影响因子：
11.4
通讯作者：
Jiao Y
中科院分区：
文献类型：
--
作者：
Park DM;Reed DW;Yung MC;Eslamimanesh A;Lencka MM;Anderko A;Fujita Y;Riman RE;Navrotsky A;Jiao Y
随着许多新兴清洁能源技术对稀土元素（REE）需求的增加，迫切需要开发有效提取和回收稀土元素的新方法。作为实现这一目标的一步，我们基因工程的好氧细菌新月柄杆菌的稀土吸附通过高密度的细胞表面显示的镧系元素结合标签（LBTs）在其S-层。LBT展示的菌株表现出增强的吸附稀土相比，缺乏LBT的细胞，高特异性的稀土元素，并与小原子半径的稀土元素的吸附偏好。吸附的Tb 3+可以有效地回收使用柠檬酸盐，与热力学形态计算预测的Tb 3+柠檬酸盐的强络合。柠檬酸盐洗脱后未观察到Tb 3+吸附能力降低，从而能够进行连续的吸附/解吸循环。LBT展示的菌株是有效的提取稀土从酸性浸出液的岩心样品收集在一个潜在的稀土矿。我们的集体研究结果表明，一个快速，有效和可逆的过程，稀土元素的吸附与潜在的工业应用，稀土富集和分离。
With the increasing demand for rare earth elements (REEs) in many emerging clean energy technologies, there is an urgent need for the development of new approaches for efficient REE extraction and recovery. As a step towards this goal, we genetically engineered the aerobic bacterium Caulobacter crescentus for REE adsorption through high-density cell surface display of lanthanide binding tags (LBTs) on its S-layer. The LBT-displayed strains exhibited enhanced adsorption of REEs compared to cells lacking LBT, high specificity for REEs, and an adsorption preference for REEs with small atomic radii. Adsorbed Tb3+ could be effectively recovered using citrate, consistent with thermodynamic speciation calculations that predicted strong complexation of Tb3+ by citrate. No reduction in Tb3+ adsorption capacity was observed following citrate elution, enabling consecutive adsorption/desorption cycles. The LBT-displayed strain was effective for extracting REEs from the acid leachate of core samples collected at a prospective rare earth mine. Our collective results demonstrate a rapid, efficient and reversible process for REE adsorption with potential industrial application for REE enrichment and separation.