Sailfish enables alignment-free isoform quantification from RNA-seq reads using lightweight algorithms.
Sailfish enables alignment-free isoform quantification from RNA-seq reads using lightweight algorithms.
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DOI:
10.1038/nbt.2862
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发表时间:
2014-05
影响因子:
46.9
通讯作者:
Kingsford, Carl
中科院分区:
文献类型:
--
作者:
Patro, Rob;Mount, Stephen M.;Kingsford, Carl
We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 times faster) without loss of accuracy. By facilitating frequent reanalysis of data and reducing the need to optimize parameters, Sailfish exemplifies the potential of lightweight algorithms for efficiently processing sequencing reads.
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