Inhibition of multidrug-resistant Acinetobacter baumannii by nonviral expression of hCAP-18 in a bioengineered human skin tissue.
Inhibition of multidrug-resistant Acinetobacter baumannii by nonviral expression of hCAP-18 in a bioengineered human skin tissue.
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DOI：
10.1038/mt.2008.289
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发表时间：
2009-03
影响因子：
12.4
通讯作者：
Allen-Hoffmann, B. Lynn
中科院分区：
文献类型：
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作者：
Thomas-Virnig, Christina L.;Centanni, John M.;Johnston, Colette E.;He, Li-Ke;Schlosser, Sandy J.;Van Winkle, Kelly F.;Chen, Ruibing;Gibson, Angela L.;Szilagyi, Andrea;Li, Lingjun;Shankar, Ravi;Allen-Hoffmann, B. Lynn
When skin is compromised, a cascade of signals initiates the rapid repair of the epidermis to prevent fluid loss and provide defense against invading microbes. During this response, keratinocytes produce host defense peptides that have antimicrobial activity against a diverse set of pathogens. Using non-viral vectors we have genetically modified the novel, non-tumorigenic, pathogen-free human keratinocyte progenitor cell line (NIKS) to express the human cathelicidin host defense peptide in a tissue-specific manner. NIKS skin tissue that expresses elevated levels of cathelicidin possesses key histological features of normal epidermis and displays enhanced antimicrobial activity against bacteria in vitro. Moreover, in an in vivo infected burn wound model, this tissue results in a two log reduction in a clinical isolate of multidrug-resistant Acinetobacter baumannii. Taken together, these results suggest this genetically engineered human tissue could be applied to burns and ulcers to counteract bacterial contamination and prevent infection.
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影响因子：
6.5
作者：
Braff, MH;Di Nardo, A;Gallo, RL
通讯作者：
Gallo, RL
影响因子：
6.5
作者：
He, YJ;Young, PK;Grinnell, F
通讯作者：
Grinnell, F
影响因子：
1.4
作者：
Muthu, Kuzhali;Melstrom, Kurt;Shankar, Ravi
通讯作者：
Shankar, Ravi
DOI：
10.1007/10_023
发表时间：
2007-01-01
期刊：
TISSUE ENGINEERING II: BASICS OF TISSUE ENGINEERING AND TISSUE APPLICATIONS
影响因子：
--
作者：
Andreadis, Stehos T.
通讯作者：
Andreadis, Stehos T.
DOI：
10.1097/01.ta.0000222975.03874.58
发表时间：
2007-04-01
影响因子：
--
作者：
Cohen, Mitchell J.;Carroll, Colleen;Shankar, Ravi
通讯作者：
Shankar, Ravi