Bacillus subtilis sigma factor sigma 29 is the product of the sporulation-essential gene spoIIG.
Bacillus subtilis sigma factor sigma 29 is the product of the sporulation-essential gene spoIIG.
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枯草芽孢杆菌 sigma 因子 sigma 29 是孢子形成必需基因 spoIIG 的产物。
DOI：
10.1073/pnas.82.12.4189
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发表时间：
1985
影响因子：
11.1
通讯作者：
W. Haldenwang
中科院分区：
文献类型：
--
作者：
J. Trempy;C. Bonamy;J. Szulmajster;W. Haldenwang
有证据表明，孢子形成必需的基因座spoIIG编码西格玛29和结构相关的蛋白质，P31。这表明至少一种特异性枯草芽孢杆菌RNA聚合酶结合蛋白在内生孢子形成中提供关键功能。spoIIG特异性RNA存在于B中。枯草杆菌培养物合成P31和sigma 29，而不合成P31和sigma 29的培养物不存在。特异于P31/sigma 29抗原决定簇的单克隆抗体检测到Spo- B中的交叉反应蛋白（P25/P21），但未检测到P31或sigma 29。在spoIIG基因座（spoIIG 41）处具有突变的枯草杆菌菌株。P25和P21的出现在该突变体中发生在P31和σ 29正常出现的时间，表明它们是同源蛋白。用携带spoIIG结构基因的质粒DNA转化spoIIG 41菌株补充Spo-表型，并导致在孢子形成期间的适当时间合成P31、σ 29、P25和P21。在大肠杆菌中，克隆的spoIIG序列编码的蛋白质与抗P31/sigma 29单克隆抗体反应，并具有真正的P31的电泳迁移率。
Evidence is presented that the sporulation-essential locus spoIIG codes for both sigma 29 and a structurally related protein, P31. This demonstrates that at least one specific Bacillus subtilis RNA polymerase binding protein provides a critical function in endospore formation. spoIIG-specific RNA is present in B. subtilis cultures that are synthesizing P31 and sigma 29 and is absent in those that are not. A monoclonal antibody specific for an antigenic determinant on P31/sigma 29 detected crossreacting proteins (P25/P21) but not P31 or sigma 29 in a Spo- B. subtilis strain with a mutation at the spoIIG locus (spoIIG41). The appearance of P25 and P21 occurs in this mutant at a time when P31 and sigma 29 would normally appear and suggests that they are homologous proteins. Transformation of the spoIIG41 strain with plasmid DNA carrying the structural gene for spoIIG complements the Spo- phenotype and results in the synthesis of P31, sigma 29, P25, and P21 at the appropriate times during sporulation. In Escherichia coli, the cloned spoIIG sequence encoded a protein that reacted with the anti-P31/sigma 29 monoclonal antibody and had the electrophoretic mobility of authentic P31.