Phosphorylation of the RNA polymerase II C-terminal domain dictates transcription termination choice
Phosphorylation of the RNA polymerase II C-terminal domain dictates transcription termination choice
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DOI：
10.1038/nsmb.1460
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发表时间：
2008-08-01
影响因子：
16.8
通讯作者：
Libri, Domenico
中科院分区：
文献类型：
--
作者：
Gudipati, Rajani Kanth;Villa, Tommaso;Libri, Domenico
隐藏不稳定转录物（CUT）是短的300-600个核苷酸（nt）的RNA聚合酶II转录物，其在酵母中被核RNA外泌体快速降解。CUT是广泛存在的，可能代表了酵母基因组中隐藏转录的最大份额。与小核仁和小核RNA类似，CUT编码基因的转录由Nrd 1复合物途径终止。我们在这里表明，这种终止模式和随后的CUT降解至关重要地依赖于RNA聚合酶II相对于转录起始位点的位置。值得注意的是，位置传感与聚合酶C-末端结构域（CTD）的磷酸化状态相关。Nrd 1复合物通过与新生RNA和CTD的相互作用被招募到染色质中，但后者的允许磷酸化状态是有效转录终止绝对需要的。我们讨论了潜在的机制，共存的隐蔽和mRNA生产转录的调节。
Cryptic unstable transcripts (CUTs) are short, 300-600-nucleotide (nt) RNA polymerase II transcripts that are rapidly degraded by the nuclear RNA exosome in yeast. CUTs are widespread and probably represent the largest share of hidden transcription in the yeast genome. Similarly to small nucleolar and small nuclear RNAs, transcription of CUT-encoding genes is terminated by the Nrd1 complex pathway. We show here that this termination mode and ensuing CUTs degradation crucially depend on the position of RNA polymerase II relative to the transcription start site. Notably, position sensing correlates with the phosphorylation status of the polymerase C-terminal domain (CTD). The Nrd1 complex is recruited to chromatin via interactions with both the nascent RNA and the CTD, but a permissive phosphorylation status of the latter is absolutely required for efficient transcription termination. We discuss the mechanism underlying the regulation of coexisting cryptic and mRNA-productive transcription.