An interstitial telomere array proximal to the distal telomere of mouse chromosome 13.
An interstitial telomere array proximal to the distal telomere of mouse chromosome 13.
复制标题
靠近小鼠 13 号染色体远端端粒的间质端粒阵列。
DOI：
10.1007/s003359900458
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Elliott,RW
中科院分区：
文献类型：
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作者：
Yen,CH;Pazik,J;Zhang,Y;Elliott,RW
来自BALB/c和C57 BL/10的小鼠DNA的λ克隆，每个克隆含有端粒六聚体阵列，通过FISH定位于接近Chr 13的端粒的区域。扩增小鼠基因组DNA与引物侧翼SSR内克隆的DNA显示了几个等位基因，这是用来分型八套RI菌株。两个λ克隆含有间质端粒阵列的等位基因版本Telrs 4，其在C57 BL/10中为495 bp，并且其包括来自共有端粒六聚体的各种序列变化。种间回交中SSR扩增产物分离与其它位点分离的比较（C57 BL/6 JEi x SPRET/Ei）F1 x SPRET/Ei与株间回交重组相比，显示重组抑制，可能与Mus spretus中Chr 13远端的核糖体DNA序列有关，（C57 BL/6 J × DBA/J）F1 × C57 BL/6 J，并与MIT F2杂交。使用第二个株间回交（ICR/Ha x C57 BL/6 Ha）F1 x C57 BL/6 Ha对雌性进行的重组分析也表明，与使用回交C57 BL/6 Ha x（ICR/Ha x C57 BL/6 Ha）F1在相同株系的雄性中进行的重组相比，重组受到抑制。位点组合顺序为D13 Mit 37-D13 Mit 30-D13 Mit 148-（D13 Rpl，2，3，4，Tel-rs 4）-D13 Mit 53-D13 Mit 196-D13 Mit 77-（D13 MU 78，35）。从杂交的数据，显然是正常的重组频率发生表明，端粒阵列约6地图单位最远的基因座Chr 13。该距离与从该地区获得的两个YAC克隆中鉴定的标记的证据一致。
Lambda clones of mouse DNA from BALB/c and C57BL/10, each containing an array of telomere hexamers, were localized by FISH to a region close to the telomere of Chr 13. Amplification of mouse genomic DNA with primers flanking SSRs within the cloned DNA showed several alleles, which were used to type eight sets of RI strains. The two lambda clones contained allelic versions of the interstitial telomere array,Telrs4, which is 495 bp in C57BL/10 and which includes a variety of sequence changes from the consensus telomere hexamer. Comparison of the segregation of the amplification products of the SSRs with the segregation of other loci in an interspecies backcross (C57BL/6JEi x SPRET/Ei) F1x SPRET/Ei shows recombination suppression, possibly associated with ribosomal DNA sequences present on distal Chr 13 inMus spretus, when compared with recombination in an interstrain backcross, (C57BL/ 6J x DBA/J) F1x C57BL/6J, and with the MIT F2intercross. Analysis of recombination in females using a second interstrain backcross, (ICR/Ha x C57BL/6Ha) F1x C57BL/6Ha, also indicates recombination suppression when compared with recombination in males of the same strains, using backcross C57BL/6Ha x (ICR/Ha x C57BL/6Ha) F1Thus, more than one cause may contribute to recombination suppression in this region. The combined order of the loci typed wasD13Mit37-D13Mit30-D13Mitl48-(D13Rpl, 2, 3, 4, Tel-rs4)-D13Mit53-D13Mitl96-D13Mit77-(D13MU78, 35). Data from crosses where apparently normal frequencies of recombination occur suggest that the telomere array is about 6 map units proximal to the most distal loci on Chr 13. This distance is consistent with evidence from markers identified in two YAC clones obtained from the region.