miRNA and Dicer in the mammalian lens: expression of brain-specific miRNAs in the lens
miRNA and Dicer in the mammalian lens: expression of brain-specific miRNAs in the lens
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DOI：
10.1007/s00418-005-0139-0
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发表时间：
2006-07-01
影响因子：
2.3
通讯作者：
Partyka, Lukasz M.
中科院分区：
文献类型：
--
作者：
Frederikse, Peter H.;Donnelly, Robert;Partyka, Lukasz M.
微小RNA（miRNAs）类似于22个核苷酸分子，其在转录后调节基因表达并控制广泛的生理和发育过程。现在有证据表明，除了抑制蛋白质翻译的能力外，miRNA还可以以组织特异性方式协调下调非常大的基因组的转录水平。在这里，我们研究了特定的miRNA和Dicer核糖核酸酶的表达，这是所需的miRNA在小鼠和大鼠晶状体的生物合成。北方印迹分析证实了晶状体中脑特异性miR-124和miR-7以及miR-125 b和let-7a的透镜表达。此外，我们提供的证据表明，肌肉特异性miR-1是不存在于透镜。我们检测了21天、6周和1年小鼠晶状体和15天大鼠透镜中的Dicer转录物，并检测了成人透镜蛋白样品中的Dicer蛋白。免疫组织化学检查晚期胚胎，出生后，和成年大鼠透镜部分确定表达的Dicer分化纤维细胞经历明显的细胞伸长的透镜内部和前上皮细胞。本研究提供的证据表明，miRNA，其中包括脑特异性形式，和Dicer在哺乳动物晶状体中表达，表明miRNA生物学的基本方面被利用的透镜在晚期胚胎和出生后的发展和成人晶状体。
Micro RNAs (miRNAs) are similar to 22 nucleotide molecules that regulate gene expression post-transcriptionally and govern a wide range of physiological and developmental processes. Evidence now indicates that miRNAs can also coordinately down-regulate transcript levels for very large groups of genes in a tissue-specific manner, in addition to their ability to suppress protein translation. Here, we examine expression of specific miRNAs and Dicer ribonuclease that is required for miRNA biogenesis in mouse and rat lenses. Northern blot analysis demonstrated lens expression of brain-specific miR-124 and miR-7 in lenses, as well as miR-125b and let-7a. In addition, we provide evidence that muscle specific miR-1 is not present in lens. We detected Dicer transcripts in 21 day, 6 week, and 1 year mouse lenses and 15 day rat lens, and detected Dicer protein in adult lens protein samples. Immunohistochemical examination of late embryonic, post-natal, and adult rat lens sections identified expression of Dicer in differentiating fiber cells that undergo pronounced cell elongation in the lens interior and anterior epithelial cells. The present study provides evidence that miRNAs, which include brain-specific forms, and Dicer are expressed in mammalian lenses, indicating that fundamental aspects of miRNA biology are utilized by the lens during late embryonic and post-natal development and in adult lenses.