Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation.
Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation.
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DOI：
10.1038/s41598-017-02297-9
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发表时间：
2017-06-16
影响因子：
4.6
通讯作者：
Khan IM
中科院分区：
文献类型：
--
作者：
Zhang Y;Morgan BJ;Smith R;Fellows CR;Thornton C;Snow M;Francis LW;Khan IM
富血小板血浆(PRP)被用来刺激急性和慢性软骨损伤的修复，尽管没有明确的证据表明这是如何实现的。损伤和病变情况下的软骨细胞经常重新表达未成熟软骨的表型生物标志物，因此组织成熟是恢复正常结构和功能的潜在途径。我们使用生长因子诱导成熟的体外模型进行比较研究，以确定PRP是否也可以诱导这种特殊形式的重塑，其特征是细胞增殖和组织僵硬增加。在PRP处理的软骨中模拟了成熟的特异性基因表达模式，软骨调制素、II型/X型胶原下调，脱碘酶II和Netrin-1上调。PRP使软骨表面细胞密度增加1.5倍(P <； 0.05)，溴脱氧尿苷掺入和增殖细胞核抗原基因表达的比例增加证实了这一点。原子力显微镜分析发现，经PRP和生长因子处理的软骨硬度增加了5倍，与赖氨酸氧化酶样蛋白-1基因表达上调10倍相关(P <； 0.001)。这些数据表明，PRP在未成熟的软骨中诱导了出生后成熟的关键方面，并为临床用于启动关节修复时评估其活性的新的生物学基础提供了基础。
Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re-express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factor-induced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin-1 upregulated. PRP increased cartilage surface cell density 1.5-fold (P < 0.05), confirmed by bromodeoxyuridine incorporation and proportionate increases in proliferating cell nuclear antigen gene expression. Atomic force microscopy analysis of PRP and growth factor treated cartilage gave a 5-fold increase in stiffness correlating with a 10-fold upregulation of lysyl oxidase like-1 gene expression (P < 0.001). These data show PRP induces key aspects of post-natal maturation in immature cartilage and provides the basis to evaluate a new biological rationale for its activity when used clinically to initiate joint repair.