An improved method for seed-bank analysis : Seedling emergence after removing the soil by sieving
An improved method for seed-bank analysis : Seedling emergence after removing the soil by sieving
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DOI：
10.2307/2390273
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发表时间：
1996-02
影响因子：
5.2
通讯作者：
G. T. Heerdt;R. Bekker;J. Bakker;G. Verweij
中科院分区：
文献类型：
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作者：
G. T. Heerdt;R. Bekker;J. Bakker;G. Verweij
1.对评估种子库大小的出苗法进行了改进，将土壤样品洗在细筛上，并将浓缩的样品铺在3-5毫米厚的灭菌盆栽堆肥上。2.与未浓缩的样品相比，该方法大大增加了出苗的数量。随后的手工分选显示，有活力的种子发芽率在81%到100%之间。3.采用这种方法，95%的幼苗将在6周内出苗。4.该方法大大减少了所需的温室空间，并使大样本量的检验成为可能。
1. The seedling emergence method for assessing the size of the seed bank is improved by washing soil samples on a fine sieve and spreading the thus concentrated samples in a 3-5 mm thick layer on sterilized potting compost. 2. The method largely increases the number of seedlings that emerge as compared to unconcentrated samples. Hand-sorting afterwards shows that the germination rates vary between 81 and 100% of the viable seeds present. 3. Ninety-five per cent of the seedlings will emerge within 6 weeks using this method. 4. The method greatly reduces the greenhouse space needed and enables examination of large sample volumes.