Potential of heterotopic fibroblasts as autologous transplanted cells for tracheal epithelial regeneration
Potential of heterotopic fibroblasts as autologous transplanted cells for tracheal epithelial regeneration
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DOI：
10.1089/ten.2007.0008
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发表时间：
2007-09-01
影响因子：
--
通讯作者：
Omori, Koichi
中科院分区：
文献类型：
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作者：
Kobayashi, Ken;Suzuki, Teruhisa;Omori, Koichi
气管上皮通过粘膜纤毛清除和调节离子和水平衡来维持呼吸道的健康。当气管被手术切除时，我们的小组已经在临床上使用人工移植物来再生气管。在这种情况下，气管上皮需要2个月的功能再生。以往的研究表明，成纤维细胞促进气管上皮再生。在这项研究中，异位成纤维细胞来源于真皮，鼻，牙龈粘膜与气管上皮细胞共培养，以评估其作为自体移植细胞的气管上皮再生的潜力。异位成纤维细胞诱导的上皮细胞在结构、纤毛发育、粘蛋白分泌、离子通道和水通道表达等方面表现出差异。这些结果表明，鼻成纤维细胞不能诱导成熟的气管上皮，真皮成纤维细胞诱导表皮样上皮。只有牙龈成纤维细胞（GFB）可以诱导形态和功能正常化的气管上皮细胞与气管成纤维细胞诱导的上皮细胞。上皮细胞增殖和迁移也被GFBs上调。这些结果表明，GFBs可用作自体移植细胞用于气管上皮再生。
The tracheal epithelium maintains the health of the respiratory tract through mucociliary clearance and regulation of ion and water balance. When the trachea is surgically removed, artificial grafts have been clinically used by our group to regenerate the trachea. In such cases, the tracheal epithelium needs 2 months for functional regeneration. Previous study has shown that fibroblasts facilitate tracheal epithelial regeneration. In this study, heterotopic fibroblasts originating from the dermis, nasal, and gingival mucosa were cocultured with tracheal epithelial cells to evaluate their potential as autologous transplanted cells for tracheal epithelial regeneration. The epithelia induced by the heterotopic fibroblasts showed differences in structure, cilia development, mucin secretion, and expression of ion and water channels. These results indicated that nasal fibroblasts could not induce mature tracheal epithelium and that dermal fibroblasts induced epidermis-like epithelium. Only the gingival fibroblasts (GFBs) could induce morphologically and functionally normalized tracheal epithelium comparable to the epithelium induced by tracheal fibroblasts. Epithelial cell proliferation and migration were also upregulated by GFBs. These results indicate that GFBs are useful as autologous transplant cells for tracheal epithelial regeneration.