Modeling of 31P-NMR spectra of magnetically oriented phospholipid liposomes:: A new analytical solution
Modeling of 31P-NMR spectra of magnetically oriented phospholipid liposomes:: A new analytical solution
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DOI：
10.1016/j.ssnmr.2005.10.009
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发表时间：
2006-06-01
影响因子：
3.2
通讯作者：
Arseniev, AS
中科院分区：
文献类型：
--
作者：
Dubinnyi, MA;Lesovoy, DM;Arseniev, AS
P-31-NMR光谱法广泛用于磷脂脂质体的研究。一种常用的生物膜模型。考虑到它们在磁场作用下会发生形变，因此有必要对脂质体的P-31-NMR谱进行正确的分析。脂质体变成椭圆形，这影响了光谱的线形。在本通讯中，我们提出了一个新的分析公式建模的P-31-NMR光谱的长形磷脂脂质体。该公式假设洛伦兹增宽函数和脂质体的精确椭圆形形状。在此基础上设计了一个P-FIT程序，用于长形脂质体实验多组分光谱的实际分析。在Mathematica环境中开发的程序的多功能性证明了一些P-31-NMR谱与不同的复杂性的模拟。(c)2005年爱思唯尔公司All rights reserved.
P-31-NMR spectroscopy is widely used for studies of phospholipid liposomes. a commonly used model of a biological membrane. For take into account that they are deformed by the magnetic field the correct analysis of P-31-NMR spectra of the liposomes it is necessary to take of the spectrometer. The liposomes become ellipsoidal and this affects the lineshape of the spectrum. In the present communication we suggest a new analytical formula for modeling of P-31-NMR spectra of the prolate phospholipid liposomes. The formula assumes a Lorentzian broadening function and exactly ellipsoidal shape of the liposomes. Based oil the formula a program called P-FIT is designed for the practical analysis of the experimental multicomponent spectra of the prolate liposomes. The versatility of the program developed in a Mathematica environment is demonstrated by simulations of a number of P-31-NMR spectra with different complexity. (c) 2005 Elsevier Inc. All rights reserved.