Spinosad resistance, esterase isoenzymes and temporal synergism in Frankliniella occidentalis (Pergande) in Australia
Spinosad resistance, esterase isoenzymes and temporal synergism in Frankliniella occidentalis (Pergande) in Australia
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DOI：
10.1016/j.pestbp.2014.07.006
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发表时间：
2014-09-01
影响因子：
4.7
通讯作者：
Gobbi, Carlotta
中科院分区：
文献类型：
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作者：
Herron, Grant A.;Gunning, Robin V.;Gobbi, Carlotta
多杀菌素在澳大利亚已被广泛用于控制西花蓟马（Pergande），但多杀菌素的有效性现在因耐药性而受到影响。在这里，我们研究了一种高度多杀菌素耐药的 F. occidentalis 菌株，以探讨酯酶是否在多杀菌素耐药性中发挥作用。通过 PAGE 电泳证实了加压的多杀菌素抗性 F. occidentalis 中酯酶活性的增强，并且估计大约比敏感菌株高出三倍。抗性菌株中的多杀菌素酯酶抑制数据显示，在多杀菌素浓度为 6.2 x 10(-7) 至 1.5 x 10(-5) M 时，存在显着的酯酶-多杀菌素结合的浓度效应。类似地，多杀菌素-胡椒基丁醚 (PBO) 抑制曲线显示出浓度效应，在 PBO 浓度为 3.3 x 之间时，抗性菌株中出现显着的酯酶-PBO 结合。 10(-5) M 和 8.4 x 10(-4) M。在敏感菌株中没有发生酯酶与多杀菌素或 PBO 的结合。生物测定结果表明，多杀菌素抗性 F. occidentalis 用环糊精复合多杀菌素与立即可用的 PBO 的 4 小时延迟释放制剂进行喷洒，结果表明多杀菌素抗性从 577 倍显着降低至 72 倍。随着进一步开发，使用类似于此处使用的延迟释放制剂的多杀菌素的 PBO 协同作用，可以为对多杀菌素耐药的 F. occidentalis 提供有效的控制。多杀菌素的时间协同作用可能被证明是控制多杀菌素耐药性西花蓟马的有效策略，其中所涉及的主要耐药机制已被证实是基于酯酶的。 (C) 2014 Elsevier Inc. 保留所有权利。
Spinosad has been widely used in Australia to control western flower thrips Frankliniella occidentalis (Pergande) but spinosad usefulness is now compromised by resistance. Here we studied a highly spinosad resistant strain of F. occidentalis to explore if esterases had a role in spinosad resistance. Enhanced esterase activity in pressured spinosad-resistant F. occidentalis was confirmed via PAGE electrophoresis and estimated to be approximately three times higher than that in a susceptible strain. Spinosad-esterase inhibition data in the resistant strain, showed a concentration effect with significant esterase-spinosad binding occurring at spinosad concentrations from 6.2 x 10(-7) to 1.5 x 10(-5) M. Similarly, a spinosad-piperonyl butoxide (PBO) inhibition curve showed a concentration effect, with significant esterase-PBO binding occurring in the resistant strain at PBO concentrations between 3.3 x 10(-5) M and 8.4 x 10(-4) M. No binding of esterase to spinosad or PBO occurred in the susceptible strain. Results of bioassays in which spinosad resistant F. occidentalis were sprayed with a 4 h delayed release formulation of cyclodextrin-complexed spinosad with immediately available PBO demonstrated that spinosad resistance was significantly reduced from 577 to 72-fold. With further development the PBO synergism of spinosad using a delayed release formulation, similar to that used here, may provide effective control for spinosad resistant F. occidentalis. Temporal synergism of spinosad may prove to be effective tactic for the control of spinosad resistant F. occidentalis where the main resistance mechanism involved has been confirmed to be esterase based. (C) 2014 Elsevier Inc. All rights reserved.