Total and specific IgE in nasal polyps is related to local eosinophilic inflammation
Total and specific IgE in nasal polyps is related to local eosinophilic inflammation
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DOI：
10.1067/mai.2001.112374
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发表时间：
2001-04-01
影响因子：
14.2
通讯作者：
van Cauwenberge, P
中科院分区：
文献类型：
--
作者：
Bachert, C;Gevaert, P;van Cauwenberge, P
背景资料：鼻息肉（NPs）以嗜酸性粒细胞性炎症为特征，常与哮喘并存，但过敏性反应和IgE在NPs发病机制中的作用尚不清楚目的：我们试图确定息肉和非息肉组织中各种过敏原的总IgE和特异性IgE与嗜酸性粒细胞性炎症标志物或皮肤试验结果之间是否存在关联。从20名具有NP的患者和20名没有NP的患者的鼻组织制备匀浆，并分析IL-5、IL-4、嗜酸性粒细胞趋化因子、白三烯（LT）C4/D4/E4、sCD 23和组胺的浓度（ELISA）。结果：NP组织中总IgE、IL-5、Eotaxin、ECP、LTC 4/D4/E4和sCD 23的浓度显著高于非息肉组织。总IgE与IL-5、ECP、LTC 4/D4/E4和sCD 23以及NP中嗜酸性粒细胞的数量显著相关。NP组1表现出不可测量的特异性IgE，NP组2选择特异性IgE，第三组表现出多克隆特异性IgE，包括对金黄色葡萄球菌肠毒素的IgE，高总IgE水平和高哮喘患病率。这些研究表明，NP中总IgE、特异性IgE水平升高与嗜酸性粒细胞炎症之间存在关联，这可能与鼻息肉病的病理生理学有关。类似地，葡萄球菌肠毒素A和B的特异性IgE的存在也指出细菌超抗原的可能作用。
Background: Nasal polyps (NPs) are characterized by eosinophilic inflammation and often coexist with asthma, However, the role of atopy and IgE in NP pathogenesis is unclearObjective: We sought to determine whether there is an association between total and specific IgE to a variety of allergens in polyp and nonpolyp tissue and markers of eosinophilic inflammation or skin test results.Methods: Homogenates were prepared from nasal tissue of 20 patients with NPs and 20 patients without NPs and analyzed for concentrations of IL-5, IL-4, eotaxin, leukotriene (LT) C4/D4/E4, sCD23, and histamine (ELISA). Eosinophil cationic protein (ECP), tryptase, and total and specific IgE for inhalant allergens and Staphylococcus aureus enterotoxins were measured (ImmunoCAP).Results: The concentrations of total IgE, IL-5, eotaxin, ECP, LTC4/D4/E4, and sCD23 were significantly higher in NP tissue compared with nonpolyp tissue. Total IgE was significantly correlated to IL-5, ECP, LTC4/D4/E4, and sCD23 and to the number of eosinophils in NPs, On the basis of the presence of specific IgE antibodies in tissue, 3 NP groups were defined. NP group 1 demonstrated no measurable specific IgE, and NP group 2 selected specific IgE, The third group demonstrated a multiclonal specific IgE, including IgE to S aureus enterotoxins, a high total IgE level, and a high prevalence of asthma.Conclusions: These studies suggest that there is an association between increased levels of total IgE, specific IgE, and eosinophilic inflammation in NPs, which may be of relevance in the pathophysiology of nasal polyposis, Similarly, the presence of specific IgE to staphylococcal enterotoxins A and B also points to a possible role of bacterial superantigens.