Salivary cortisol concentrations and behavior in a population of healthy dogs hospitalized for elective procedures.
Salivary cortisol concentrations and behavior in a population of healthy dogs hospitalized for elective procedures.
复制标题
唾液皮质醇的浓度和行为在健康狗群中，住院治疗。
DOI：
10.1016/j.applanim.2012.08.007
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发表时间：
2012-11
影响因子：
2.3
通讯作者：
Dreschel, Nancy A.
中科院分区：
文献类型：
--
作者：
Hekman, Jessica P.;Karas, Alicia Z.;Dreschel, Nancy A.
识别住院兽医患者的严重压力可能会改善治疗结果和福利。为了评估压力水平，在研究 1 中，我们收集了 28 只择期手术前住院的健康狗的唾液皮质醇样本和行为参数。狗被分为两组；低皮质醇 (LC) 和高皮质醇 (HC)，基于皮质醇浓度分布（< 或 ≥ 0.6 µg/dL）。我们基于与唾液皮质醇浓度最密切相关的三种行为（头休息、气喘吁吁和舔嘴唇）构建了压力研究工具（SRT）。在研究 2 中，我们收集了另外 39 只狗的唾液皮质醇样本，评估了行为/皮质醇关系，将每只狗分配到 LC 或 HC 组，并测试了 SRT 预测唾液皮质醇的能力。研究1（0.43 µg/dL，0.33至1.00 µg/dL）和研究2狗（0.41 µg/dL，0.28至0.52 µg/dL）之间的中位（四分位数范围）唾液皮质醇浓度没有差异。在每项研究中，LC 犬的唾液皮质醇中位浓度显着低于 HC 犬（P ≤ 0.001）； （研究 1 LC：0.38 µg/dL，（0.19 至 0.44），n = 19，HC：2.0 µg/dL，（1.0 至 2.8），n = 9，研究 2 LC：0.35 µg/dL，（0.25 至 0.48），n = 28，HC：0.89 µg/dL，（0.66 至 1.4），n = 7）。在研究 1 中，发现三种行为与唾液皮质醇浓度相关。头部休息的持续时间与唾液皮质醇呈负相关（ρ = -0.60，P = 0.001），喘气和舔嘴唇与皮质醇呈正相关（分别为ρ = 0.39，P = 0.04和0.30，P = 0.05），头部休息（p = 0.001）和气喘（p = 0.003）也与皮质醇相关。 LC/HC 组分配。在研究 2 中，狗的三种行为与唾液皮质醇浓度相关（但不显着）；在这三者中，只有头部休息与 LC/HC 组分配显着相关 (P = 0.03)。当应用于研究 2 的 20 分钟而不是 2 分钟的行为数据时，来自研究 1 的 SRT 可以有效预测唾液皮质醇浓度。此外，我们注意到，与未镇静的狗相比，在测量前超过 6 小时的右美托咪定和布托啡诺镇静与较低的唾液皮质醇浓度显着相关（P = 0.05）。我们的工作为最终构建一个评级工具提供了支持，该工具利用特定行为的存在或不存在来识别住院狗中较高的唾液皮质醇浓度，这可能与较大的心因性压力水平有关。未来研究压力对狗的影响及其在临床情况下的缓解的工作可以通过研究参数的组合来进行，并且应该考虑镇静剂可能的有益作用。
Identification of severe stress in hospitalized veterinary patients may improve treatment outcomes and welfare. To assess stress levels, in Study 1, we collected salivary cortisol samples and behavioral parameters in 28 healthy dogs hospitalized prior to elective procedures. Dogs were categorized into two groups; low cortisol (LC) and high cortisol (HC), based on the distribution of cortisol concentrations (< or ≥ 0.6 µg/dL). We constructed a stress research tool (SRT) based on three behaviors, (head resting, panting and lip licking) that were most strongly related to salivary cortisol concentrations. In Study 2, we collected salivary cortisol samples from 39 additional dogs, evaluated behavior/cortisol relationships, assigned each dog to an LC or HC group, and tested the ability of the SRT to predict salivary cortisol. Median (interquartile range) salivary cortisol concentrations were not different between Study 1 (0.43 µg/dL, 0.33 to 1.00 µg/dL) and Study 2 dogs (0.41 µg/dL, 0.28 to 0.52 µg/dL). The median salivary cortisol concentration was significantly lower (P ≤ 0.001) in LC versus HC dogs in each study; (Study 1 LC: 0.38 µg/dL, (0.19 to 0.44), n = 19, HC: 2.0 µg/dL, (1.0 to 2.8), n = 9, and Study 2 LC: 0.35 µg/dL, (0.25 to 0.48), n = 28, HC: 0.89 µg/dL, (0.66 to 1.4), n = 7). In Study 1, three behaviors were found to be associated with salivary cortisol concentrations. Duration of head resting was negatively associated with salivary cortisol (ρ = −0.60, P = 0.001), panting and lip licking were positively associated with cortisol (ρ = 0.39, P = 0.04, and 0.30, P = 0.05, respectively), Head resting (p = 0.001) and panting (p = 0.003) were also associated with LC/HC group assignment. In Study 2 dogs, the three behaviors correlated (but not significantly) with salivary cortisol concentration; of the three, only head resting was significantly associated with LC/HC group assignment (P = 0.03). The SRT derived from Study 1 was effective at prediction of salivary cortisol concentrations when applied to 20 min but not 2 min of behavioral data from Study 2. Additionally, we note that dexmedetomidine and butorphanol sedation more than 6 h prior to measurement was found to be significantly (P = 0.05) associated with lower salivary cortisol concentrations when compared to unsedated dogs. Our work offers support for eventual construction of a rating tool that utilizes the presence or absence of specific behaviors to identify higher salivary cortisol concentrations in dogs subjected to hospitalization, which may be tied to greater psychogenic stress levels. Future work to investigate the effects of stress on dogs and its mitigation in clinical situations may be approached by studying a combination o f parameters, and should consider the possible beneficial effects of sedatives.
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影响因子：
2.3
作者：
Dreschel, NA;Granger, DA
通讯作者：
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影响因子：
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