Hydrogen sulfide alleviates aluminum toxicity in germinating wheat seedlings.
Hydrogen sulfide alleviates aluminum toxicity in germinating wheat seedlings.
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DOI：
10.1111/j.1744-7909.2010.00946.x
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发表时间：
2010-06
影响因子：
11.4
通讯作者：
Hua Zhang;Zhu-Qin Tan;Lan-Ying Hu;Song-hua Wang;Jian-ping Luo;Russell L. Jones
中科院分区：
文献类型：
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作者：
Hua Zhang;Zhu-Qin Tan;Lan-Ying Hu;Song-hua Wang;Jian-ping Luo;Russell L. Jones
研究了硫化氢(H(2)S)对铝胁迫下小麦种子萌发和幼苗生长的保护作用。结果表明，高浓度的AlCl3对小麦萌发和幼苗生长均有抑制作用。当浓度为30 mm ol/L时，AlCl3的发芽率下降约50%，对幼苗生长的抑制作用更为明显。小麦种子在H(2)S供体NaHS中预培养，在0.3 mmol/L的最佳浓度下以剂量依赖的方式减轻AlCl3诱导的胁迫。通过证明NaHS处理后内源H(2)S/Hs(-)的水平增加，证实NaHS减轻Al(3+)胁迫的作用可归因于H(2)S/Hs(-)。此外，其他含硫钠盐对缓解Al(3+)胁迫效果不明显。NaHS处理显著提高了萌发种子在Al(3+)胁迫下的淀粉酶和酯酶活性，维持了较低的丙二醛和H(2)O(2)水平。NaHS处理提高了愈创木酚过氧化物酶、抗坏血酸过氧化物酶、超氧化物歧化酶和过氧化氢酶的活性，降低了脂肪氧合酶的活性。NaHS处理也降低了AlCl3处理种子对Al(3+)的吸收。综上所述，这些结果表明，H(2)S能够提高小麦种子的抗氧化能力，从而缓解Al(3+)胁迫。
Protective role of hydrogen sulfide (H(2)S) on seed germination and seedling growth was studied in wheat (Triticum) seeds subjected to aluminum (Al(3+)) stress. We show that germination and seedling growth of wheat is inhibited by high concentrations of AlCl(3). At 30 mmol/L AlCl(3) germination is reduced by about 50% and seedling growth is more dramatically inhibited by this treatment. Pre-incubation of wheat seeds in the H(2)S donor NaHS alleviates AlCl(3)-induced stress in a dose-dependant manner at an optimal concentration of 0.3 mmol/L. We verified that the role of NaHS in alleviating Al(3+) stress could be attributed to H(2)S/HS(-) by showing that the level of endogenous H(2)S increased following NaHS treatment. Furthermore, other sodium salts containing sulfur were ineffective in alleviating Al(3+) stress. NaHS pretreatment significantly increased the activities of amylases and esterases and sustained much lower levels of MDA and H(2)O(2) in germinating seeds under Al(3+) stress. Moreover, NaHS pretreatment increased the activities of guaiacol peroxidase, ascorbate peroxidase, superoxide dismutase and catalase and decreased that of lipoxygenase. NaHS pretreatment also decreased the uptake of Al(3+) in AlCl(3)-treated seed. Taken together these results suggest that H(2)S could increase antioxidant capability in wheat seeds leading to the alleviation of Al(3+) stress.