Dual inhibitory pathways link antral somatostatin and histamine secretion in human, dog, and rat stomach.
Dual inhibitory pathways link antral somatostatin and histamine secretion in human, dog, and rat stomach.
复制标题
双重抑制途径将人、狗和大鼠胃中的窦生长抑素和组胺分泌联系起来。
DOI：
10.1016/0016-5085(95)90645-2
复制
发表时间：
1995
期刊：
影响因子：
29.4
通讯作者：
Makhlouf,GM
中科院分区：
文献类型：
--
作者：
Vuyyuru,L;Schubert,ML;Harrington,L;Arimura,A;Makhlouf,GM
背景与目的 窦组胺的分泌和功能尚不清楚。本研究的目的是表征人类、狗和大鼠胃窦释放组胺的机制，并确定组胺是否会影响生长抑素和胃泌素的分泌。方法采用放射免疫分析法测定灌注胃窦段的生长抑素、胃泌素和组胺分泌情况。结果 灌注硫哌丁胺（H3 拮抗剂）可增加所有三个物种的生长抑素并减少胃泌素和组胺的分泌；灌注(r)-α-甲基组胺（H3 激动剂）具有相反的效果。该模式表明，内源性组胺通过 H3 受体发挥作用，对生长抑素分泌产生抑制性旁分泌影响，进而调节胃泌素分泌。灌流生长抑素抗体可增加组胺分泌；这种增加不受胃泌素拮抗剂 L-365,260 的影响，这意味着它不是由胃泌素同时增加介导的，而是由连接生长抑素和组胺的抑制途径的抑制介导的。单独灌注乙酰甲胆碱以及在 H3 激动剂或拮抗剂存在下证实存在连接生长抑素和组胺的相互抑制途径。结论 人类、狗和大鼠的胃窦组胺通过相互抑制性旁分泌途径与胃窦生长抑素相关，从而放大生长抑素的调节影响。
Background & Aims The secretion and function of antral histamine are not known. The aims of this study were to characterize the mechanisms of histamine release from the gastric antrum of humans, dogs, and rats and to determine whether histamine can influence the secretion of somatostatin and gastrin. Methods Somatostatin, gastrin, and histamine secretion from superfused antral segments was measured using radioimmunoassay. Results Superfusion with thioperamide (H3antagonist) increased somatostatin and decreased gastrin and histamine secretion in all three species; superfusion with (r)-α-methylhistamine (H3agonist) had the opposite effect. The pattern implied that endogenous histamine, acting via H3receptors, exerts an inhibitory paracrine influence on somatostatin secretion, which in turn regulates gastrin secretion. Superfusion with somatostatin antibody increased histamine secretion; the increase was not affected by the gastrin antagonist L-365,260, implying that it was not mediated by the concurrent increase in gastrin but by suppression of an inhibitory pathway linking somatostatin and histamine. Superfusion with methacholine alone and in the presence of either the H3agonist or antagonist confirmed the existence of reciprocal inhibitory pathways linking somatostatin and histamine. Conclusions Antral histamine in humans, dogs, and rats is linked to antral somatostatin via reciprocal inhibitory paracrine pathways that serve to amplify the regulatory influence of somatostatin.