A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury
A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury
复制标题
一种用于评估缺血/再灌注损伤期间内源性过氧化氢的近红外荧光探针
作者：
Xu Runfeng;Wang Yue;You Huiyan;Zhang Liangwei;Wang Yunqing;Chen Lingxin
过氧化氢（H2 O2）作为活性氧（ROS）的主要成分，在机体的正常生理过程中起着重要作用。H_2O_2爆发也发生在缺血/再灌注（I/R）过程中，并引起一系列生理和病理损伤。因此，重要的是确定H2 O2的浓度波动。在这里，我们开发了一种比率荧光探针，Cy-ArB，它对H2 O2表现出高选择性和灵敏度。该探针的荧光响应是由硼酸酯与H2 O2反应引发的，该过程释放出具有线粒体示踪能力的近红外七甲川菁荧光团。因此，该探针可用于实时监测线粒体呼吸链中的H2 O2波动。最后，我们探讨了H2 O2在细胞和在体内的波动，在I/R过程中使用的探针Cy-ArB。我们的实验结果证明，我们的探针是一个潜在的候选人的临床手术前评估。
Hydrogen peroxide (H2O2), as a major component of reactive oxygen species (ROS), plays an important role in normal physiological processes. A H2O2 burst also occurs in the ischemia/reperfusion (I/R) process and causes a series of physiological and pathological injuries. Therefore, it is important to determine concentration fluctuations of H2O2. Here we develop a ratiometric fluorescent probe, Cy-ArB, which shows high selectivity and sensitivity toward H2O2. The fluorescence response of the probe is triggered by the reaction of borate esters with H2O2, and this process releases a near-infrared heptamethine cyanine fluorophore which has the ability of mitochondrial tracing. Hence, the probe can be used for real-time monitoring of H2O2 fluctuations in the mitochondrial respiration chain. Finally, we explore the fluctuations of H2O2 in cells and in vivo during the I/R process using the probe Cy-ArB. The results of our experiments prove that our probe is a potential candidate for clinical surgery pre-evaluation.