MOLECULAR-BASIS OF EMETINE RESISTANCE IN CHINESE-HAMSTER OVARY CELLS - ALTERATION IN 40S RIBOSOMAL-SUBUNIT
MOLECULAR-BASIS OF EMETINE RESISTANCE IN CHINESE-HAMSTER OVARY CELLS - ALTERATION IN 40S RIBOSOMAL-SUBUNIT
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DOI：
10.1016/0092-8674(77)90140-4
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发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
SIMINOVITCH, L
中科院分区：
文献类型：
--
作者：
GUPTA, RS;SIMINOVITCH, L
对蛋白合成抑制剂emetine的抗性的分子基础在来自正常和emetine抗性（EmtR）[中国仓鼠卵巢，CHO]突变体的无细胞蛋白合成提取物中进行了检查。突变体细胞提取物中的蛋白质合成对雌激素的抑制作用具有抗性。当从野生型和突变体细胞系的提取物被分馏成上清液（S-100）和多聚核糖体馏分和混合在不同的组合，耐embryonic与突变体多聚核糖体馏分。将野生型和突变型多聚核糖体进一步分级分离成40 S和60 S核糖体亚基，并将它们以各种组合与来自野生型细胞的S-100级分混合，表明突变型细胞对embryonic的抗性涉及40 S核糖体亚基的改变。还在体细胞杂种中检查了EmtR的行为。EmtR的研究。体内和体外的EmtS杂交细胞系表明，EmtR对EmtS是表型隐性的，这与遗传改变的核糖体位置一致。
The molecular basis of resistance to the protein synthesis inhibitor emetine was examined in cell-free, protein-synthesizing extracts derived from normal and emetine-resistant (EmtR) [Chinese hamster ovary, CHO] mutants. Protein synthesis in extracts of the mutant cells is resistant to the inhibitory action of the emetin. When extracts from a wild-type and mutant cell line were fractionated into supernatant (S-100) and polyribosome fractions and mixed in different combinations, resistance to emetine was associated with the mutant polyribosome fraction. Further fractionation of wild-type and mutant polyribosomes into 40S and 60S ribosomal subunits and mixing them in various combinations with an S-100 fraction from the wild-type cell indicates that resistance of mutant cells to emetine involves an alteration in the 40S ribosomal subunit. The behavior of EmtR was also examined in somatic cell hybrids. Studies of EmtR .times. EmtS hybrid cell lines in vivo and in vitro show that EmtR is phenotypically recessive to EmtS, which is consistent with the ribosomal location of the genetic change.