Molecular and cytogenetic characterization of a metallothionein gene of Drosophila.
Molecular and cytogenetic characterization of a metallothionein gene of Drosophila.
复制标题
果蝇金属硫蛋白基因的分子和细胞遗传学特征。
DOI：
10.1093/genetics/112.3.493
复制
发表时间：
1986
期刊：
影响因子：
3.3
通讯作者：
Lastowski-Perry,D
中科院分区：
文献类型：
--
作者：
Maroni,G;Otto,E;Lastowski-Perry,D
以果蝇（Drosophilamelanogaster）的cDNA为探针，从果蝇基因组文库中分离到一个含有金属硫蛋白基因的染色体DNA片段。对1543个碱基对（bp）的片段进行测序，发现包括被一个小内含子中断的cDNA序列。几条证据表明，果蝇中存在金属硫蛋白基因（MTN）的单拷贝;任何其他相关基因，如果它们存在的话，必须足够不同，以至于它们不能被我们的探针检测到，即使在降低严格性的杂交条件下。经原位杂交和缺失定位，Mtnis定位于第3条染色体的右臂85 E10 -15区域。在转录起始的表观位点上游300个碱基内，有几个短的间隔非常类似于被认为负责哺乳动物系统中金属调节的12-bp片段。
A chromosomal DNA segment containing the metallothionein gene was isolated from a genomic library ofDrosophila melanogasterusing a previously characterized cDNA of this species as a probe. A segment of 1543 base pair (bp) was sequenced and found to include the cDNA sequence interrupted by one small intron. Several lines of evidence indicate that there is a single copy of the metallothionein gene (Mtn) in Drosophila; any other related genes, if they occur, must be sufficiently different that they are not detectable by our probe, even under hybridization conditions of reduced stringency. According toin situhybridization and deletion mapping,Mtnis located in the right arm of the third chromosome in region 85E10-15. Within 300 bases upstream of the apparent site of transcription initiation, there are several short intervals very similar to the 12-bp segments considered to be responsible for metal regulation in mammalian systems.
DOI：
10.1073/pnas.81.23.7318
发表时间：
1984-12
影响因子：
11.1
作者：
G. Stuart;P. Searle;Howard Y. CHENt;R. L. BRINSTERt;R. Palmiter
通讯作者：
G. Stuart;P. Searle;Howard Y. CHENt;R. L. BRINSTERt;R. Palmiter
金属硫蛋白和果蝇细胞对镉中毒的抵抗力。
DOI：
--
发表时间：
1985
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
A. Debec;R. Mokdad;M. Wegnez
通讯作者：
M. Wegnez