A cell-wall protein SRPP provides physiological integrity to the Arabidopsis seed
A cell-wall protein SRPP provides physiological integrity to the Arabidopsis seed
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DOI：
10.1007/s10265-018-01083-6
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发表时间：
2019-01
影响因子：
2.8
通讯作者：
H. Uno;Natsuki Tanaka-Takada;Momoko Hattori;Mayu Fukuda;M. Maeshima
中科院分区：
文献类型：
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作者：
H. Uno;Natsuki Tanaka-Takada;Momoko Hattori;Mayu Fukuda;M. Maeshima
种子和根毛保护蛋白（SRPP）在种子和根毛中表达，定位于细胞壁，并参与细胞壁的完整性。我们分析了SRPP的功能丧失突变体，重点是角果和种子。这些rpp-1植株以高比率产生暗褐色皱缩种子。这些缺陷的srpp-1种子的发芽率低于6%，而表面上正常的srpp-1种子的发芽率为83%。严重表型种子的生产率取决于生长条件。在低湿度条件下培养的rpp-1植株，其缺陷率为73%，显著高于正常湿度条件下的缺陷率。授粉后第7天可检测到角果和种子的缺陷，角果的顶端区域以高频率显示严重的表型。与胚、种皮或瓣（心皮）特异性启动子控制下的SRPP基因互补部分挽救了表型，而使用SRPP启动子互补完全挽救了表型。此外，SRPP的过表达增强了耐热性。在50 °C下处理种子2小时后，35 S启动子过表达的种子的发芽率增加到野生型种子的两倍。在相同条件下，nosrpp-1种子萌发。这些结果表明，SRPP是必要的生产正常可行的种子在应力条件下的角果。SRPP基因的修饰可能提高种子的完整性。
Seed and root hair protective protein (SRPP) is expressed in seeds and root hairs, localized in the cell wall, and involved in cell wall integrity. We analyzed a loss-of-function mutant ofSRPP, focusing on siliques and seeds. Thesrpp-1plants generated dark brown shrunken seeds at a high rate. The germination rate of these defect seeds ofsrpp-1was less than 6%, although apparently normalsrpp-1seeds germinated at a rate of 83%. The production ratio of severe phenotypic seeds was dependent on the growth conditions. When thesrpp-1plants were cultivated at low humidity, the defect ratio was 73%, which was significantly higher than that at normal humidity. Defects of the silique and seeds could be detected on day 7 after pollination and the apical region of the siliques displayed a severe phenotype at a high frequency. Complementation with anSRPPgene under the control of promoters specific to the embryo, seed coat, or valve (carpel) partially rescued the phenotype, and complementation using theSRPPpromoter fully rescued the phenotype. Furthermore, overexpression ofSRPPenhanced the thermotolerance. After the treatment of seeds at 50 °C for 2 h, the germination rate of the seeds from overexpression with the 35S promoter increased to levels twice that of the wild-type seeds. Under the same conditions, nosrpp-1seeds germinated. These results indicate that SRPP is essential for the production of normal viable seeds in siliques under stress conditions. It is possible that modification of theSRPPgene improves seed integrity.