Facile preparation of a novel iminodisuccinate modified chitin and its excellent properties as a silver bioadsorbent and antibacterial agent.
Facile preparation of a novel iminodisuccinate modified chitin and its excellent properties as a silver bioadsorbent and antibacterial agent.
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DOI：
10.1016/j.carbpol.2023.120793
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发表时间：
2023-03
影响因子：
11.2
通讯作者：
Yafeng Ge;Yong Zhang;Jia-Shun Yang;Wang-Yang Ye;Hui-Min Gao;Jun-Zhi Liu;Qisheng Bao;Wei-Li Jiang
中科院分区：
文献类型：
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作者：
Yafeng Ge;Yong Zhang;Jia-Shun Yang;Wang-Yang Ye;Hui-Min Gao;Jun-Zhi Liu;Qisheng Bao;Wei-Li Jiang
以蟹壳为原料，采用一步法制备了一种新型亚氨基二磺酸修饰甲壳素。接枝度为1.46、脱乙酰度为47.68%的ICH对银离子Ag(I)的最大吸附量为2572.41 mg/g。ICH还表现出良好的选择性和可重用性。Freundlich等温线模型对吸附效果较好，拟一阶和拟二阶动力学模型均拟合良好。表征结果表明，ICH具有优异的Ag(I)吸附性能应归因于其疏松的孔隙结构和附加的官能团接枝分子。此外，载银ICH （ICH- ag）对6种典型病原菌（大肠杆菌、铜绿假单胞菌、产气肠杆菌、鼠伤寒沙门菌、金黄色葡萄球菌和单核增生李斯特菌）的抑菌效果显著，其90%最小抑菌浓度范围为0.426 ~ 0.685 mg/mL。对银的释放、微细胞形态和宏基因组分析的进一步研究表明，ICH-Ag吸附后形成了许多银纳米颗粒，其抑菌机制包括破坏细胞膜和扰乱细胞内代谢。研究了甲壳素基生物吸附剂的制备、金属的去除和回收以及抗菌剂的生产对蟹壳废弃物的耦合处理方案。
A novel iminodisuccinate modified chitin (ICH) was prepared using crab shells via a one-step facile procedure. The ICH with grafting degree of 1.46 and deacetylation degree of 47.68 % possessed maximum adsorption capacity of 2572.41 mg/g for silver ions (Ag(I)).The ICH also exhibited good selectivity and reusability. The adsorption followed better with the Freundlich isotherm model, while fitted well with both the Pseudo-first-order and Pseudo-second-order kinetics models. The characteristical results showed that the excellent Ag(I) adsorption capability of ICH should be attributed to both looser porous microstructure as well as additional functional groups-grafting molecular. Moreover, the Ag-loaded ICH (ICH-Ag) showed remarkable antibacterial properties against six typical pathogenic bacteria strains (Escherichia coli,Pseudomonas aeruginosa, Enterobacter aerogenes, Salmonella typhimurium, Staphylococcus aureus,and Listeria monocytogenes), with the corresponding 90 % minimal inhibitory concentrations ranged 0.426–0.685 mg/mL. Further study on the silver release, microcell morphology, and metagenomic analysis suggested that many Ag nanoparticles were formed after the Ag(I) adsorption, and the antibacterial mechanisms of the ICH-Ag involved both cell membranes destruction and intracellular metabolism disturbing. This research presented a coupling solution of crab shell wastes treatment with chitin-based bioadsorbents preparation, metal removal and recovery, as well as antibacterial agent production.