Clinical Significance of Human Kallikrein7 Gene Expression in Colorectal Cancer
Clinical Significance of Human Kallikrein7 Gene Expression in Colorectal Cancer
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DOI：
10.1245/s10434-010-1132-y
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发表时间：
2010-11-01
影响因子：
3.7
通讯作者：
Kusunoki, Masato
中科院分区：
文献类型：
--
作者：
Inoue, Yasuhiro;Yokobori, Takehiko;Kusunoki, Masato
背景人激肽释放酶相关肽酶（KLK）被认为是癌症中重要的预后生物标志物。本研究旨在探讨KLK 7基因在结直肠癌中的表达及其与结直肠癌临床病理因素的关系。KLK 7信使RNA（mRNA）的表达在9个CRC癌细胞系通过实时聚合酶链反应进行了检查。同时检测136例大肠癌组织和136例癌旁正常组织中KLK 7 mRNA的表达水平。9个细胞系中有6个表达KLK 7基因。癌组织中KLK 7 mRNA表达水平显著高于正常组织。多因素分析显示，KLK 7 mRNA表达水平是一个独立的预后因素，尤其是在肝转移中。我们提供的证据表明，KLK 7 mRNA表达与结直肠癌患者的预后相关，特别是在肝转移中。
Background. The human kallikrein-related peptidases (KLK) are considered important prognostic biomarkers in cancer. The aim of the current study is to demonstrate gene expression of KLK7 in colorectal cancer (CRC) and to correlate the relative KLK7 expression level with clinicopathological factors of CRC.Methods. KLK7 messenger RNA (mRNA) expression was examined in nine CRC cancer cell lines by real-time polymerase chain reaction. The expression levels of KLK7 mRNA in cancerous tissues (n = 136) and paired normal tissues (n = 136) of CRC patients were also examined.Results. Six of the nine cell lines expressed the KLK7 gene. KLK7 mRNA expression levels in cancer tissues were significantly higher than those in normal tissues. Multivariate analysis revealed that the KLK7 mRNA expression level in cancer was an independent prognostic factor, especially in liver metastasis.Conclusions. We provide evidence suggesting that KLK7 mRNA expression is correlated with prognosis in CRC patients, especially in liver metastasis.