Glucose transporters are abundant in cells with "occluding" junctions at the blood-eye barriers.
Glucose transporters are abundant in cells with "occluding" junctions at the blood-eye barriers.
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葡萄糖转运蛋白在血眼屏障处具有“闭塞”连接的细胞中含量丰富。
DOI：
10.1073/pnas.87.11.4261
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发表时间：
1990
影响因子：
11.1
通讯作者：
Perry,G
中科院分区：
文献类型：
--
作者：
Harik,SI;Kalaria,RN;Whitney,PM;Andersson,L;Lundahl,P;Ledbetter,SR;Perry,G
我们利用人红细胞葡萄糖转运蛋白 C 末端的单克隆和多克隆抗体进行免疫细胞化学研究，研究了“红细胞/脑”葡萄糖转运蛋白在人和大鼠眼中的分布。我们在视网膜、视神经和虹膜的微血管内皮中发现了强烈的免疫细胞化学染色，但在脉络膜、睫状体、巩膜和其他眼眶后组织的微血管中没有发现。此外，我们还发现视网膜色素上皮、睫状体上皮和虹膜后上皮有明显的免疫细胞化学染色。所有那些对葡萄糖转运蛋白染色强烈的内皮和上皮细胞的共同特征是存在“闭塞”细胞间连接，它构成了血眼屏障的解剖基础。我们认为，高密度的葡萄糖转运蛋白是具有屏障特征的上皮细胞和内皮细胞的生化伴随物，至少在对葡萄糖有高代谢需求的组织中是这样。
We studied the distribution of the "erythroid/brain" glucose transporter protein in the human and rat eye by immunocytochemistry with monoclonal and polyclonal antibodies to the C terminus of the human erythrocyte glucose transporter. We found intense immunocytochemical staining in the endothelium of microvessels of the retina, optic nerve, and iris but not in microvessels of the choroid, ciliary body, sclera, and other retro-orbital tissues. In addition, we found marked immunocytochemical staining of retinal pigment epithelium, ciliary body epithelium, and posterior epithelium of the iris. The common feature of all those endothelial and epithelial cells that stained intensely for the glucose transporter is the presence of "occluding" intercellular junctions, which constitute the anatomical bases of the blood-eye barriers. We propose that a high density of the glucose transporter is a biochemical concomitant of epithelial and endothelial cells with barrier characteristics, at least in tissues that have a high metabolic requirement for glucose.
影响因子：
4.1
作者：
KASANICKI, MA;CAIRNS, MT;BALDWIN, SA
通讯作者：
BALDWIN, SA
辣根过氧化物酶在虹膜血管中的单向转运机制。
影响因子：
4.4
作者：
Raviola,G;Butler,JM
通讯作者：
Butler,JM