Reversibility of the adverse effects of 1-bromopropane exposure in rats.
Reversibility of the adverse effects of 1-bromopropane exposure in rats.
复制标题
DOI：
10.1093/toxsci/kfm245
复制
发表时间：
2007-12
期刊：
影响因子：
--
通讯作者：
Shameema Banu;S. Ichihara;F. Huang;Hidenori Ito;Y. Inaguma;K. Furuhashi;Y. Fukunaga;Qiang-yi Wang;J. Kitoh;H. Ando;F. Kikkawa;G. Ichihara
中科院分区：
文献类型：
--
作者：
Shameema Banu;S. Ichihara;F. Huang;Hidenori Ito;Y. Inaguma;K. Furuhashi;Y. Fukunaga;Qiang-yi Wang;J. Kitoh;H. Ando;F. Kikkawa;G. Ichihara
以前的实验表明，1-溴丙烷（1-BP），氯氟烃的替代品，是神经毒性和抑制精子在睾丸。在这里，我们研究了1-BP在大鼠中毒性作用的可逆性。将雄性Wistar大鼠分成三个相等的组，每组24只，并通过吸入暴露于0、400或1000 ppm的1-BP 6周（8小时/天，7天/周）。在6周暴露结束时以及暴露结束后4周和14周，每组处死8只大鼠，以评估恢复过程。我们研究了精子数量、活力、形态学和睾丸组织病理学，以及血压、皮肤温度和后肢肌肉力量。在暴露于1000 ppm 6周结束时（0周恢复期），睾丸重量、附睾重量、精子计数和活力较低，形态异常精子增加，生精细胞显示弥漫性变性。这些变化在14周恢复时未显示完全恢复，但前列腺和精囊重量除外，其恢复至对照值。在400 ppm时，0周恢复期保留的精子细胞增加，4周恢复期恢复正常。暴露于1000 ppm产生持续减少后肢肌肉力量在14周的恢复，而皮肤温度和血压的正常化后，注意到短暂的变化。我们的研究表明，1-BP对精子发生的影响是剂量依赖性的;低剂量暴露抑制精子和精子依赖性器官重量减少，这些变化是短暂的，而较高剂量的1000 ppm 1-BP引起持续耗竭的生精细胞。
Previous experiments indicated that 1-bromopropane (1-BP), an alternative to chloroflurocarbons, is neurotoxic and inhibits spermiation in the testis. Here we investigated the reversibility of the toxic effects of 1-BP in rats. Male Wistar rats were divided into three equal groups of 24 each and exposed by inhalation to 0, 400 or 1000 ppm of 1-BP for 6 weeks (8 hrs/day, 7 days/week). Eight rats from each group were sacrificed at the end of 6 weeks exposure, and at 4 and 14 weeks after the end of exposure, to assess the recovery processes. We studied sperm count, motility, morphology and testicular histopathology, as well as blood pressure, skin temperature and hindlimb muscle strength. At the end of 6 weeks of exposure to 1000 ppm (0 week recovery), testicular weight, epididymal weight, sperm count and motility were low, morphologically abnormal sperm were increased and spermatogenic cells showed diffuse degeneration. These changes did not show full recovery at 14 weeks recovery, with the exception of the prostate and seminal vesicular weights, which recovered back to control values. At 400 ppm, increased retained spermatids at 0 week recovery returned to normal at 4 weeks recovery. Exposure to 1000 ppm produced sustained reduction of hindlimb muscle strength at 14 weeks recovery, whereas normalization of the skin temperature and blood pressure was noted after transient changes. Our study showed that the effect of 1-BP on spermatogenesis is dose-dependent; low exposure inhibited spermiation and hormone-dependent organ weight reduction and these changes were transient, while a higher dose of 1000 ppm 1-BP caused persistent depletion of spermatogenic cells.