Immunological and chemical analysis of P6, the carboxyl-terminal fragment of HIV P15.
Immunological and chemical analysis of P6, the carboxyl-terminal fragment of HIV P15.
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HIV P15 的羧基末端片段 P6 的免疫学和化学分析。
DOI：
10.1089/aid.1987.3.253
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发表时间：
1987
影响因子：
1.5
通讯作者：
Sarngadharan,MG
中科院分区：
文献类型：
--
作者：
Veronese,FD;Rahman,R;Copeland,TD;Oroszlan,S;Gallo,RC;Sarngadharan,MG
HIV基因组的第一个开放阅读框架被确定为gag基因。由该基因编码的蛋白质是作为氨基末端蛋白的p17、作为中间肽的p24和作为羧基末端的p15。已经开发出识别p15片段上的抗原决定簇的单克隆抗体，并命名为M35/2F 8。该单克隆抗体在放射性测序p15，p6的羧基末端产物，并在确定该蛋白质和氨基末端产物p7之间的切割位点中起了重要作用。通过免疫亲和层析，还可以从HIV裂解物中纯化p6，并从HIV感染的细胞中纯化所有含有p6的多聚蛋白。这些结果使我们对HIV gag基因的组成和加工过程有了更多的了解。
The first open reading frame of the HIV genome has been identified as thegaggene. The proteins encoded by this gene are p17 as the amino-terminal protein, p24 as the middle peptide, and p15 as the carboxyl-terminal end. A monoclonal antibody recognizing an antigenic determinant on a fragment of p15 has been developed and designated M35/2F8. This monoclonal has been instrumental in radiosequencing the carboxyl-terminal product of p15, p6, and in determining the cleavage site between this protein and the amino-terminal product, p7. By immunoaffinity chromatography it was also possible to purify p6 from HIV lysates and all p6 containing polyproteins from HIV-infected cells. These results gave more insight into the composition and processing of the HIVgaggene.