Characterization of pro-opiocortin, a precursor to opioid peptides and corticotropin.
Characterization of pro-opiocortin, a precursor to opioid peptides and corticotropin.
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阿片皮质素原（阿片肽和促肾上腺皮质激素的前体）的表征。
DOI：
10.1073/pnas.75.2.669
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发表时间：
1978
影响因子：
11.1
通讯作者：
S. Udenfriend
中科院分区：
文献类型：
--
作者：
M. Rubinstein;S. Stein;S. Udenfriend
用胰蛋白酶消化先前在大鼠垂体提取物中检测到的阿片样物质活性的高分子量（约30，000）前体（前阿片皮质素），并通过高效反相色谱法分离所得肽混合物。阿片样物质活性的峰在九肽β-LPH（61-69）的位置洗脱，其也是通过胰蛋白酶消化β-促脂素或β-内啡肽获得的相同片段。这表明这种蛋白质是内啡肽和甲硫氨酸脑啡肽的前体。在对应于β-LPH（61-69）的Leu 5类似物的位置未检测到活性，因此排除了垂体提取物中Leu-脑啡肽的β-促脂素样前体的可能性。前阿片皮质素和β-促脂素在大鼠垂体提取物中的含量相当，约为10 pmol/mg组织。
The high molecular weight (approximately 30,000) precursor to opioid activity (pro-opiocortin) previously detected in extracts of rat pituitary was digested with trypsin and the resulting peptide mixture was resolved by high-performance reverse-phase chromatography. A peak of opioid activity was eluted at the position of the nonapeptide beta-LPH (61-69), which was also the same fragment obtained by trypsin digestion of betas-lipotropin or beta-endorphin. This identified the protein as a precursor to the endorphins and Met-enkephalin. No activity was detected in the position corresponding to the Leu5 analog of betas-LPH (61-69), thus ruling out the possibility of a beta-lipotropin-like precursor to Leu-enkephalin in pituitary extracts. Pro-opiocortin and beta-lipotropin are present in rat pituitary extracts in comparable amounts, approximately 10 pmol/mg of tissue.