A singularly narrow 29 nm aerosol size standard based on the ΦX174 bacteriophage

A singularly narrow 29 nm aerosol size standard based on the ΦX174 bacteriophage
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DOI:
10.1016/j.jaerosci.2021.105949
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发表时间:
2022-01-11
影响因子:
4.5
通讯作者:
de la Mora, Juan Fernandez
de la Mora, Juan Fernandez
中科院分区:
环境科学与生态学3区
文献类型:
--
作者:
de la Mora, Juan Fernandez

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Phi X174噬菌体的电喷雾样品的高分辨率迁移率分析揭示了以平均迁移率直径d(p)= 28.9 nm为中心的异常窄的尺寸分布(相对直径半峰全宽低于0.7%)。这些二十面体颗粒可在浓度超过10(9)/mL的溶液中商购获得。在此,它们被简单地透析到10 mM醋酸铵水溶液中,然后在双极电喷雾中雾化。
High resolution mobility analysis of an electrosprayed sample of the Phi X174 bacteriophage reveals an exceptionally narrow size distribution (relative diameter full width at half maximum below 0.7%) centered at a mean mobility diameter d(p) = 28.9 nm. These icosahedral particles are commercially available in solution at concentrations in excess of 10(9)/mL. Here they are simply dialyzed into 10 mM aqueous ammonium acetate, before being atomized in a bipolar electrospray.