Unique roles of microRNA140 and its host gene WWP2 in cartilage biology.
Unique roles of microRNA140 and its host gene WWP2 in cartilage biology.
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DOI：
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发表时间：
2008-10
影响因子：
1.9
通讯作者：
Y. Nakamura;X. He;T. Kobayashi;Y. L. Yan;J. Postlethwait;M. Warman
中科院分区：
文献类型：
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作者：
Y. Nakamura;X. He;T. Kobayashi;Y. L. Yan;J. Postlethwait;M. Warman
发展。然后，用功能丧失和功能获得的方法在体内研究miR-140的功能。在斑马鱼中，使用反义寡核苷酸(Morpholinos：MOS)耗尽miR-140显著减小了咽软骨的大小。当共注射miR140双链时，这种表型被挽救。当miR-140过表达时，咽软骨的大小显著增加。然而，我们还不知道miR-140调控面部软骨发育的机制。根据miR-140在斑马鱼软骨中的表达模式，我们推测软骨的主要调控因子Sox9
development. Then, the function of miR-140 was investigated in vivo using loss-of-function and gain-of-function approaches. In zebrafish, depletion of miR-140 using anti-sense oligonucleotides (Morpholinos: MOs) significantly decreased the size of the pharyngeal cartilage. This phenotype was rescued when miR140 duplex was co-injected. When miR-140 was over-expressed, the size of the pharyngeal cartilage was significantly increased. However, we do not yet know the mechanism by which miR-140 regulates facial cartilage development. Based on the expression pattern of miR-140 in zebrafish cartilage, we hypothesized that the transcription factor Sox9, a master regulator of cartilage