Activated clearance of a biotinylated macromolecular MRI contrast agent from the blood pool using an avidin chase
Activated clearance of a biotinylated macromolecular MRI contrast agent from the blood pool using an avidin chase
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DOI：
10.1021/bc034064l
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发表时间：
2003-09-01
影响因子：
4.7
通讯作者：
Choyke, PL
中科院分区：
文献类型：
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作者：
Kobayashi, H;Kawamoto, S;Choyke, PL
造影剂的增强特性取决于其在体内的药代动力学。在大分子造影剂的情况下，在第一次注射后，血液池持续增强，限制了在同一疗程中重复注射的机会。如果可以故意关闭血池内的增强，大分子造影剂就可以用来确定血管体积和血管通透性，这两个特性可能有助于研究血管生成。在目前的研究中，亲和素-生物素系统被偶联到基于树枝状大分子的大分子磁共振造影剂上，以将增强从血池切换到肝脏。由于亲和素会使造影剂迅速滞留在肝脏中，所以注射亲和素后2分钟内血池就会清除。该系统可应用于所有树枝状大分子大分子磁共振造影剂的血容量和血管通透性研究。此外，它还允许在单次磁共振实验中重复注射造影剂和“亲和素开关”。
The enhancement characteristics of a contrast agent are dependent on its pharmacokinetics within the body. In the case of macromolecular contrast agents, prolonged enhancement of the blood pool is seen after the first dose, limiting opportunities for repeated injection in the same session. If the enhancement within the blood pool could be intentionally switched off, the macromolecular contrast agents could be used both to define blood volume and vessel permeability, properties that could be useful in studying angiogenesis. In the current study, the avidin-biotin system was coupled to a dendrimer-based macromolecular MRI contrast agent to switch enhancement from the blood pool to the liver. Because avidin causes rapid trapping of the contrast agent in the liver, the blood pool cleared within 2 min of the injection of avidin. This system can be applied to all dendrimer-based macromolecular MRI contrast agents to investigate blood volume and vascular permeability. Moreover, it permits the repeated injection of the contrast agent and the "avidin switch" during a single MR experiment.