Killing of isogeneic erythrocytes by neutrophils in ginbuna crucian carp (Carassius auratus langsdorfii)
Killing of isogeneic erythrocytes by neutrophils in ginbuna crucian carp (Carassius auratus langsdorfii)
复制标题
银鲫 (Carassius auratus langsdorfii) 中性粒细胞对同基因红细胞的杀伤作用
DOI：
10.1006/fsim.1998.0156
复制
发表时间：
1998
影响因子：
4.7
通讯作者：
T. Nakanishi
中科院分区：
文献类型：
--
作者：
U. Fischer;M. Ototake;T. Nakanishi
描述了鱼类中性粒细胞对同基因红细胞的细胞毒活性。用密度梯度离心分离克隆鲫鱼无致敏的富中性粒细胞白细胞，与等基因红细胞进行培养。用四甲基联苯胺（TMB）测定血红蛋白释放到上清液中，从而测定同基因红细胞的裂解。所获得的细胞毒性水平与效应细胞组分中粒细胞的含量和效应细胞与靶细胞的比例成正比。只有在中性粒细胞与靶红细胞直接接触后，才会发生同基因红细胞的溶解。当粒细胞和红细胞在聚碳酸酯膜分隔的同一培养基中培养时，没有观察到红细胞溶解，因此只允许交换上清而不允许交换细胞。因此，红细胞溶解不是由于粒细胞释放到上清液中的可溶性因子。当红细胞和中性粒细胞在琼脂糖中固定在一起培养时，只有在红细胞与中性粒细胞接触的情况下，显微镜下才能观察到红细胞溶解。因此，中性粒细胞似乎能够引起组织相容细胞的裂解。
Cytotoxic activity of fish neutrophils against isogeneic erythrocytes is described. Unsensitised neutrophil-rich leucocytes from clonal ginbuna crucian carp, separated by density gradient centrifugation, were cultured with isogeneic erythrocytes. Lysis of isogeneic erythrocytes was determined by measuring the release of haemoglobin into the supernatant using tetra-methylbenzidine (TMB). The level of cytotoxicity obtained was proportional to both the content of granulocytes in effector cell fractions and the effector to target cell ratio. Lysis of isogeneic erythrocytes occurred only after direct contact of neutrophils with target erythrocytes. No erythrolysis was observed when granulocytes and erythrocytes were cultured in the same medium separated by a polycarbonate membrane, thus allowing exchange of only supernatant but not cells. Erythrolysis was therefore not due to a soluble factor released into the supernatant by the granulocytes. When erythrocytes and neutrophils were cultured together by immobilising them in agarose, erythrolysis was only observed microscopically in cases where erythrocytes were in contact with neutrophils. Thus, neutrophils appear able to cause lysis of histocompatible cells.