Phenomics enables measurement of complex responses of developing animals to global environmental drivers
Phenomics enables measurement of complex responses of developing animals to global environmental drivers
复制标题
表型组学能够测量发育中的动物对全球环境驱动因素的复杂反应
DOI：
10.1016/j.scitotenv.2022.159555
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发表时间：
2023
影响因子：
9.8
通讯作者：
Tills O
中科院分区：
文献类型：
--
作者：
Tills O
表型组学为高维表型分析提供了技术进步，促进了对生理性能的快速、高通量评估，并已被证明在包括药物发现和食品安全在内的全球研究挑战中具有宝贵价值。然而，这一迅速发展的学科在很大程度上仍然无法满足日益紧迫的挑战，即评估生物体对全球变化驱动因素的功能敏感性。在这里，我们调查了生态上重要的海洋无脊椎动物对多种环境驱动因素的反应，使用能量代理特征（EPTS），这是一种新的方法，用于测量视频上捕获的复杂表型，作为波动像素值中不同时间频率的能量水平谱。我们成像的三个发展阶段的共同prawnPalaemon serratus在不同的盐度和温度，并测量EPT和心率，一个主要的代理的生理表现在外温动物跨阶段。在频率特异性能量水平上，温度、发育阶段和盐度之间存在显著的交互作用。尽管心脏活动是EPT谱的重要贡献者，但治疗相互作用与EPT上观察到的相互作用不同，突出了EPT的其他表型驱动因素。升高的温度导致EPT谱向更高频率信号的偏移，表明表型组内资源的重新分配。使用非线性降维，我们询问的EPT光谱在高维空间中的响应。我们发现了复杂的发育阶段特异性敏感性，突出了表型反应的复杂性，以及使用具有预先选择的性状的单变量方法来评估对多种全球环境驱动因素的反应的局限性。EPT是一种高维的、可转移的表型分型方法，因此与解决传统表型分型方法的当前局限性高度相关，这些方法用于评估对全球变化驱动因素的生物敏感性。我们预测，EPTS将成为一个重要的工具，不分青红皂白的表型，物种之间，发展阶段和实验设计转移。
Phenomics offers technological advances for high-dimensional phenotyping, facilitating rapid, high-throughput assessment of physiological performance and has proven invaluable in global research challenges including drug discovery and food security. However, this rapidly growing discipline has remained largely inaccessible to the increasingly urgent challenge of assessing organismal functional sensitivity to global change drivers. Here, we investigate the response of an ecologically important marine invertebrate to multiple environmental drivers using Energy Proxy Traits (EPTs), a new approach for measuring complex phenotypes captured on video as a spectrum of energy levels across different temporal frequencies in fluctuating pixel values. We imaged three developmental stages of the common prawnPalaemon serratusat different salinities and temperatures, and measured EPTs and heart rate, a major proxy of physiological performance in ectotherms present across stages. Significant interactions were detected between temperature, developmental stage and salinity in frequency-specific energy levels. Despite cardiac activity being a significant contributor to the EPT spectra, treatment interactions were different from those observed on EPTs, highlighting additional phenotypic drivers of EPTs. Elevated temperature resulted in a shift of the EPT spectra towards higher frequency signals, indicating a reallocation of resources within the phenome. Using a non-linear dimensionality reduction, we interrogated the responses of EPT spectra in high-dimensional space. We discovered complex developmental-stage specific sensitivities, highlighting both the complexity of phenotypic responses, and the limits of using univariate approaches with pre-selected traits to assess responses to multiple global environmental drivers. EPTs are a high-dimensional, transferrable method of phenotyping, and are therefore highly relevant to addressing the current limitations of traditional methods of phenotyping applied to assessing biological sensitivity to drivers of global change. We predict that EPTs will become an important tool for indiscriminate phenotyping, transferrable between species, developmental stages and experimental designs.