Transplantation of Achilles tendon treated with bone morphogenetic protein 7 promotes meniscus regeneration in a rat model of massive meniscal defect.
Transplantation of Achilles tendon treated with bone morphogenetic protein 7 promotes meniscus regeneration in a rat model of massive meniscal defect.
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DOI：
10.1002/art.38099
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发表时间：
2013-11
影响因子：
--
通讯作者：
Sekiya, Ichiro
中科院分区：
文献类型：
--
作者：
Ozeki, Nobutake;Muneta, Takeshi;Koga, Hideyuki;Katagiri, Hiroki;Otabe, Koji;Okuno, Makiko;Tsuji, Kunikazu;Kobayashi, Eiji;Matsumoto, Kenji;Saito, Hirohisa;Saito, Tomoyuki;Sekiya, Ichiro
本研究旨在探讨骨形态发生蛋白7（BMP-7）是否诱导大鼠肌腱异位软骨形成，以及BMP-7处理的肌腱移植是否促进肌腱再生。此外，我们分析了宿主和供体细胞对大鼠肌腱移植后愈合过程的相对贡献。将BMP-7原位注射到大鼠跟腱中，并对组织学结果和基因谱进行评价。将注射有1 μg BMP-7的跟腱移植到大鼠的骨缺损中。分别于术后4、8、12周对再生半月板和关节软骨进行评价。将LacZ转基因大鼠的跟腱移植到野生型大鼠的跟腱缺损处，反之亦然。将BMP-7注射到大鼠跟腱中诱导肌腱细胞的纤维软骨细胞分化，并改变肌腱组织的胶原基因谱，使其更接近肌腱组织。将大鼠跟腱移植到桡骨缺损处可增加桡骨尺寸。接受用BMP-7处理的肌腱的大鼠具有半月板基质，其表现出番红O和II型胶原染色增加，并且表现出关节软骨降解的延迟。使用LacZ转基因大鼠，我们确定半月板的再生来自供体和宿主细胞的贡献。我们的研究结果表明，BMP-7诱导大鼠肌腱异位软骨形成。BMP-7处理的跟腱移植促进半月板再生并防止大面积半月板缺损大鼠模型中的软骨退变。大鼠跟腱中的天然细胞有助于跟腱再生。
This study was undertaken to examine whether bone morphogenetic protein 7 (BMP-7) induces ectopic cartilage formation in the rat tendon, and whether transplantation of tendon treated with BMP-7 promotes meniscal regeneration. Additionally, we analyzed the relative contributions of host and donor cells on the healing process after tendon transplantation in a rat model. BMP-7 was injected in situ into the Achilles tendon of rats, and the histologic findings and gene profile were evaluated. Achilles tendon injected with 1 μg of BMP-7 was transplanted into a meniscal defect in rats. The regenerated meniscus and articular cartilage were evaluated at 4, 8, and 12 weeks. Achilles tendon from LacZ-transgenic rats was transplanted into the meniscal defect in wild-type rats, and vice versa. Injection of BMP-7 into the rat Achilles tendon induced the fibrochondrocyte differentiation of tendon cells and changed the collagen gene profile of tendon tissue to more closely approximate meniscal tissue. Transplantation of the rat Achilles tendon into a meniscal defect increased meniscal size. The rats that received the tendon treated with BMP-7 had a meniscus matrix that exhibited increased Safranin O and type II collagen staining, and showed a delay in articular cartilage degradation. Using LacZ-transgenic rats, we determined that the regeneration of the meniscus resulted from contribution from both donor and host cells. Our findings indicate that BMP-7 induces ectopic cartilage formation in rat tendons. Transplantation of Achilles tendon treated with BMP-7 promotes meniscus regeneration and prevents cartilage degeneration in a rat model of massive meniscal defect. Native cells in the rat Achilles tendon contribute to meniscal regeneration.
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DOI：
10.1016/j.arthro.2004.06.038
发表时间：
2004-11-01
影响因子：
4.7
作者：
Ahn, JH;Wang, JH;Yoo, JC
通讯作者：
Yoo, JC
DOI：
10.1302/0301-620x.74b6.1447257
发表时间：
1992-11-01
影响因子：
--
作者：
KOHN, D;WIRTH, CJ;WULKER, N
通讯作者：
WULKER, N
影响因子：
2.8
作者：
Chubinskaya, Susan;Kawakami, Mamoru;Rueger, David C.
通讯作者：
Rueger, David C.
影响因子：
5.3
作者：
Garrett, WE;Swiontkowski, MF;DeRosa, GP
通讯作者：
DeRosa, GP
影响因子：
5.4
作者：
Matsuda, Akio;Morita, Hideaki;Abe, Jun
通讯作者：
Abe, Jun