COMMITMENT OF MOUSE FIBROBLASTS TO ADIPOCYTE DIFFERENTIATION BY DNA TRANSFECTION
COMMITMENT OF MOUSE FIBROBLASTS TO ADIPOCYTE DIFFERENTIATION BY DNA TRANSFECTION
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DOI：
10.1126/science.2470149
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发表时间：
1989-05-05
期刊：
影响因子：
56.9
通讯作者：
WISE, LS
中科院分区：
文献类型：
--
作者：
CHEN, S;TEICHER, LC;WISE, LS
Cells of the mouse cell line 3T3-F442A can be induced by various hormones to differentiate into adipocytes, whereas cells of 3T3-C2, a subclone of 3T3, cannot. However, transfection of DNA from uninduced 3T3-F422A cells into 3T3-C2 cells permits recovery of 3T3-C2 transfectants that differentiate into adipocytes in the presence of insulin. DNA isolated from human fat tissue, when transfected into 3T3-C2 mouse cells, also gives rise to mouse transfectants that are induced to differentiate into adipocytes by the addition of insulin. Apparently, transfection of a transregulatory gene (or genes) from 3T3-F442A or human fat cells into 3T3-C2 cells is sufficient to commit 3T3-C2 cells to adipocyte differentiation.
