MATING TYPES AND MATING INDUCING SUBSTANCES IN EUPLOTES-OCTOCARINATUS
MATING TYPES AND MATING INDUCING SUBSTANCES IN EUPLOTES-OCTOCARINATUS
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DOI：
10.1002/jez.1402370113
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发表时间：
1986-01-01
影响因子：
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通讯作者：
KUHLMANN, HW
中科院分区：
文献类型：
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作者：
HECKMANN, K;KUHLMANN, HW
本文报道了一种淡水纤毛虫（Euplotes octocarinatus）与髌骨纤毛虫（e.p atella）密切相关的10种不同交配类型的细胞之间的复杂反应模式。两种不同类型的细胞混合后，在大多数组合中，经过约3小时的等待期，会形成异型和同型对。在少数组合中，只形成两种混合类型中的一种的同型对。同型和异型对都经历减数分裂和完全偶联。杂交实验表明，这10种交配类型是由4个共显性等位基因（mt1、mt2、mt3和mt4）决定的，这些等位基因控制着4种交配诱导信号物质或激素（G1、G2、G3和G4）的产生。这些物质被分泌到培养基中，并诱导某些其他交配类型的成熟细胞转化为能够结合并形成共轭对的细胞。我们认为，细胞转化为准备结合的细胞是由gamone分子与gamone特异性受体结合触发的，成熟细胞通常暴露所有的gamone受体，除了它们自己产生的受体。
The complex pattern of reactions between cells of ten different mating types of Euplotes octocarinatus, a fresh water ciliate closely related to E. patella, is reported. Mixture of cells of two different types leads in the majority of combinations to the formation of both heterotypic and homotypic pairs after a waiting period of about 3 hours. In a few combinations only homotypic pairs of one of the two mixed types are formed. Both homotypic and heterotypic pairs undergo meiosis and complete conjugation. Cross breeding experiments show that the ten mating types are determined by four codominant alleles (mt1, mt2, mt3, and mt4) that control the production of four mating-inducing signal-substances or gamones (G1, G2, G3, and G4). These substances are secreted into the medium and induce mature cells of certain other mating types to transform into cells that are able to unite and form conjugant pairs. We suggest that the transformation of the cells into cells ready for conjugation is triggered by the binding of gamone molecules to gamone-specific receptors and that mature cells usually exposed receptors for all gamones except the ones they produce themselves.