Requirement of NMDA receptor reactivation for consolidation and storage of nondeclarative taste memory revealed by inducible NR1 knockout
Requirement of NMDA receptor reactivation for consolidation and storage of nondeclarative taste memory revealed by inducible NR1 knockout
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DOI：
10.1111/j.1460-9568.2005.04257.x
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发表时间：
2005-08-01
影响因子：
3.4
通讯作者：
Tsien, JZ
中科院分区：
文献类型：
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作者：
Cui, ZZ;Lindl, KA;Tsien, JZ
我们采用一种可诱导的、可逆的和区域特异性的基因敲除技术，利用条件性味觉厌恶记忆范式，研究了小鼠非陈述性、海马区非陈述性记忆的不同阶段(获取、巩固和存储以及提取)对皮质NMDAR的需求。在这里，我们表明，在学习或学习后巩固阶段，而不是在提取阶段，皮质NMDAR的临时敲除会导致在为期1个月的味觉记忆保持测试中出现严重的表现缺陷。更重要的是，我们发现长期非陈述性味觉记忆的巩固和存储需要大脑皮层NMDAR的重新激活。因此，NMDAR在学习后阶段的动态参与使我们假设NMDAR重新激活介导的突触重入强化对于克服突触蛋白周转固有的不稳定效应以及实现大脑中非陈述性记忆的巩固和存储至关重要。
We employed an inducible, reversible and region-specific gene knockout technique to investigate the requirements for cortical NMDA receptors (NMDAR) during the various stages (acquisition, consolidation and storage, and retrieval) of nondeclarative, hippocampal-independent memory in mice using a conditioned taste aversion memory paradigm. Here we show that temporary knockout of the cortical NMDAR during either the learning or postlearning consolidation stage, but not during the retrieval stage, causes severe performance deficits in the 1-month taste memory retention tests. More importantly, we found that the consolidation and storage of the long-term nondeclarative taste memories requires cortical NMDAR reactivation. Thus, the dynamic engagement of the NMDAR during the postlearning stage leads us to postulate that NMDAR reactivation-mediated synaptic re-entry reinforcement is crucial for overcoming the destabilizing effects intrinsic to synaptic protein turnover and for achieving consolidation and storage of nondeclarative memories in the brain.