Stress Relaxation Measurement of Fibroblast Cells with Atomic Force Microscopy
Stress Relaxation Measurement of Fibroblast Cells with Atomic Force Microscopy
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DOI：
10.1143/jjap.46.5552
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发表时间：
2007-08
影响因子：
1.5
通讯作者：
T. Okajima;Masaru Tanaka;S. Tsukiyama;T. Kadowaki;Sadaaki Yamamoto;M. Shimomura;H. Tokumoto
中科院分区：
文献类型：
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作者：
T. Okajima;Masaru Tanaka;S. Tsukiyama;T. Kadowaki;Sadaaki Yamamoto;M. Shimomura;H. Tokumoto
我们用原子力显微镜（AFM）测量了小鼠成纤维细胞NIH3T3细胞的应力松弛，原子力显微镜使用锋利的硅针尖和半径为1.5 μm的二氧化硅珠作为压头。在NIH3T3细胞中，在0.4nN的小初始加载力下清楚地观察到加载力的衰减，并且很好地拟合于拉伸指数函数而不是单一指数函数。两种压头的拉伸指数参数均为0.5，表明在NIH 3T3细胞中观察到的应力松弛由多个松弛过程组成。本报告中描述的时域AFM技术使我们能够直接测量活细胞在毫秒到秒的范围内的弛豫过程。
We measured the stress relaxation of mouse fibroblast NIH3T3 cells with an atomic force microscope (AFM) using a sharp silicon tip and a silica bead with a radius of ∼1 µm as an indenter. The decay of loading force was clearly observed in NIH3T3 cells at a small initial loading force of ∼0.4 nN and was well fitted to the stretched exponential function rather than to a single exponential function. The stretching exponent parameter was ∼0.5 for both indenters, indicating that the stress relaxation observed in NIH3T3 cells consisted of multiple relaxation processes. The time-domain AFM technique described in this report allows us to measure directly the relaxation process of living cells in a range from milliseconds to seconds.