Streptococcus gordonii comCDE (competence) operon modulates biofilm formation with Candida albicans
Streptococcus gordonii comCDE (competence) operon modulates biofilm formation with Candida albicans
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DOI：
10.1099/mic.0.000010
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发表时间：
2015-02-01
期刊：
影响因子：
2.8
通讯作者：
Nobbs, Angela H.
中科院分区：
文献类型：
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作者：
Jack, Alison A.;Daniels, Debbie E.;Nobbs, Angela H.
白色念珠菌是一种多形性真菌，它与早期口腔表面的定殖者戈登链球菌形成混合生物膜。群体感应(QS；细胞间信号传递)的激活促进了这些微生物对非物种生物膜的发育，但QS在混合物种群落中的作用尚不清楚。戈登链霉菌中的comCDE基因编码一个传感器调节系统(ComDE)，该系统被comc基因产物(CSP)激活，并调节QS调节基因的表达。与戈登葡萄球菌DL1野生型与白色念珠菌的生物膜相比，戈登葡萄球菌Delta comCDE或Delta Comc突变株与白色念珠菌的双物种生物膜显示出更高的生物量。Delta comCDE突变体双物种生物膜尤其含有更多的胞外DNA(EDNA)，并且可以用DNase I或蛋白酶处理来分散。外源CSP补充了戈登链霉菌Delta Comc的转化缺陷以及Delta Comc-C的转化缺陷。白念珠菌生物被膜表型。纯化的CSP不影响白念珠菌菌丝的形成，但抑制单种白念珠菌生物被膜的形成。结果表明，戈登葡萄球菌comCDE QS-系统调节EDNA的产生和白色念珠菌在双种生物膜中的掺入。
Candida albicans is a pleiomorphic fungus that forms mixed species biofilms with Streptococcus gordonii, an early colonizer of oral cavity surfaces. Activation of quorum sensing (QS; intercellular signalling) promotes nnonospecies biofilm development by these micro-organisms, but the role of QS in mixed species communities is not understood. The comCDE genes in S. gordonii encode a sensor regulator system (ComDE), which is activated by the comC gene product (CSP, competence stimulating peptide) and modulates expression of QS-regulated genes. Dual species biofilms of S. gordonii Delta comCDE or Delta comC mutants with C. albicans showed increased biomass compared to biofilms of S. gordonii DL1 wild-type with C. albicans. The Delta comCDE mutant dual species biofilms in particular contained more extracellular DNA (eDNA), and could be dispersed with DNase I or protease treatment. Exogenous CSP complemented the S. gordonii Delta comC transformation deficiency, as well as the Delta comC-C. albicans biofilm phenotype. Purified CSP did not affect C. albicans hyphal filament formation but inhibited monospecies biofilm formation by C. albicans. The results suggest that the S. gordonii comCDE QS-system modulates the production of eDNA and the incorporation of C. albicans into dual species biofilms.